program to quantitate 2D spots from NCBI - (Dec/05/2006 )

my professor wants me to quantitate 2D spots and says some database is available from NCBI. i searched and searched ad couldnt find any. any suggestions?

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ok not NCBI any other online free tool???? please!

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the request is still unclear to me

do you want to qualify your spot by Mw/pI parameters?

or to measure intensity of spots in gel or blot?


these are two totally different approaches
to measure intensity use a scan of your gel/blot and use a qualified program such as Imagemaster/Melanie

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thank you very much for your reply! yes, i want to quantify that intensity of the spots, sorry for the lack of clearness

i will search for the program on google.

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We use PDQuest from BioRad. Complete version isn´t free but I´m not sure if on BioRad´s website there´s a free trial for a month.

http://www.bio-rad.com/B2B/BioRad/product/...vel=Lit+Request

I have also found these, they offer demo versions but not the complete program completely free:

Delta

2D_analysis

By the way, this is a very complete site with lots of useful links if working with bidimensional gels:

http://www.expasy.ch/ and here it has software to download but not complete versions:
http://www.expasy.ch/melanie/2DImageAnalysisViewer.html

Hey, I think I have found it now (it´s this one FREE, isn´t it??)!!

http://open2dprot.sourceforge.net/index.html

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thank you so much Pumuki!!!!!!!
i have tried Imagemaster/Melanie, but they dont offer a complete version online, and i am not able to select spots... i dont know if it:s me not understanding something there...
however now im downloading 30 day free trial from biorad... thank you so much! i hope i can figure it out.

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it seems it needs wondows XP so i need to bring my labtop to download it. here in the lab all computers are windows 2000.

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Hi Kathy,
I haven´t worked with Imagemaster or Melanie so I can´t help you, sorry. I hope that the free trial from BioRad is the complete version and is useful for you. I think they have manuals to download, if not, I could send you PDQuest manual.
good luck

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thanks for the nice links, especially

http://open2dprot.sourceforge.net/index.html

was new to me; did you ever work with this kind of program?

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Hi kosmodrom
I´ve never worked with this program. I´ve only worked a bit with QuantityOne and PDQuest (our lab bought them).
I found open2dprot the other day, it´s also new to me. It seems a good program, and I think it was the only one whose complete version is free

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