anti-gfp antibodies - (Feb/20/2008 )

hey ,
anyone know if anti-gfp antibodies will detect EGFP in a western?

thnaks

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Depends. Find out which antibody you want to use- find out what is the recognition sequence for it and then checjk if that recognition sequence appears in your EGFP.

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yes, they work.

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I've used anti-eGFP a couple of times... it can be done as long at the antibody that you're using isn't total crap.

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polyclonal anti-gfp normally detect true GFP-derivatives which means E/GFP, E/CFP, E/YFP etc

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