Dialysis ? - (Feb/06/2008 )
Hi Guys
Can dialysis be use to exchange the buffer ? I want to exchange the buffer of my protein from the sod phos. to hepes buffer. sample vol is 3 ml. For how long we should dialyze it and also how many changes we should do ??
Thanks
-veerubiotech-
QUOTE (veerubiotech @ Feb 6 2008, 05:36 PM)
Hi Guys
Can dialysis be use to exchange the buffer ? I want to exchange the buffer of my protein from the sod phos. to hepes buffer. sample vol is 3 ml. For how long we should dialyze it and also how many changes we should do ??
Thanks
Can dialysis be use to exchange the buffer ? I want to exchange the buffer of my protein from the sod phos. to hepes buffer. sample vol is 3 ml. For how long we should dialyze it and also how many changes we should do ??
Thanks
Yes, dialysis is used to exchange buffer
3 changes of 500ml buffer.
1st buffer for 6 hr
2nd buffer overnight
3rd buffer 4-6hr.
Hope this may help.
-Minnie Mouse-