Glycoproteins on SDS-PAGE - (Aug/10/2004 )

Hi Everybody

Can anybody help me on how to selectively stain glycoproteins on SDS-PAGE gel or on PVDF/Nylon membrane??

Thanx
Cell biol

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Hi!
You might want to try lectins, wich usually bind specifically to glycoproteins.
Here's a link you might find interesting... http://www.probes.com/handbook/tables/0418.html
some nice figures...
Hope I could help!

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You may want to try this prodcut from Pierce: GelCode Glycoprotein Staining Kit

http://www.piercenet.com/Objects/View.cfm?...ly&ID=02051020#

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There is a florescent dye called ProQ emerald-but its quite expensive but very sensitive.

Also you can use another staining technique in the lab using tymol and sulfuric acid that stains glycoproteins red on the gel. Ref: Racusen, Anal Biochem 474-476.

As for the lectins they bind to glycoproteins on your membrane you can get them linked to different molecules the one I use are linked to horseradish peroxidase. EY labs in New York sell them,I attached a pdf catologue, they do niffy kits with several different glycoproteins with different specifies. They have distributors world wide.


Sorry bout the spelling in a rush

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