Whats the best media to get the fastest E.coli growth at a low cost? (only conce - (Dec/01/2005 )

I dont need to isolate plasmids, or produce a protein.. I just need to produce the highest cell biomass of E. coli possible in 8 hours. I start with a 200mL shakeflask inoculum, then add the culture to a 9.8L fermenter (10L total). The fermentation will occur in a total of 8 hours..

What media would you recommend to get the highest cell biomass? Right now im thinking LB (1% tryptone, 0.5% yeast extract, 0.5% NaCl) but surely theres a better alternative out there.

Id use superbroth (3.5/2/0.5% of those 3 ingredients respectively) but its too expensive, same with terrific broth (1.2/2.4/phosphates).

Any suggestions? Should I add phosphates to my media? (KH2PO4/K2HPO4)? Any other component suggestion that I should use? (soy-peptone, glucose, lactose, etc) Should I go with the Glucose M9Y media? Or should I use some wierd % of trpytone/yeast extract/nacl like 1.4/0.8/0.7%)

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You should use Mach1 cells (Invitrogen). They grow very fast compared to most other coli strains (they are not K-12). Aeration is critical. Use baffled shaker flasks or good bubblers. You might think about an oxygen enriched atmosphere.

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I dont have a choice in my aeration, method of fermentation, strain, etc

Only thing i can control is media..
What would you guys recommend?
Please anyone?

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couldn't you possibly make the superbroth yourself? or terrific broth, for that matter. the recipes are pretty simple. i purify protein a lot, and make my own superbroth.

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Adding some glycerol used to double the cell density, for over night cultures, but I do not know if eight hour would be enough to get the full effect, and I have only used it in 1-3 ml cultures in tubes, never in larger volumes.
And, I don't remember the name of the broth, or the recipe. It probably were a recipe once, but over the years it developed into - throw a little glycerol into the tube if hunch told me I could use some extra cells.

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