can I freeze the gel slice for protein sequencing - (Mar/24/2005 )
I am doing photolabeling now, and I have to send the protein band (in a SDS-PAGE gel slice 6mmx3mm) to another place to sequence it. Can I freeze the gel slice and put it in a dry ice box? The protein ( with tritiated ligand covalently bond) will be OK with freezing?
And, another question, should I wash away the residue SDS from the gel slice so the band won't diffuse? Will the protein diffuse away from the gel if I don't wash away SDS? The SDS concentration is 2% in loading buffer and 0.1% in running buffer.
Thanks
-bigworm-
QUOTE (bigworm @ Mar 24 2005, 12:30 PM)
I am doing photolabeling now, and I have to send the protein band (in a SDS-PAGE gel slice 6mmx3mm) to another place to sequence it. Can I freeze the gel slice and put it in a dry ice box? The protein ( with tritiated ligand covalently bond) will be OK with freezing?
And, another question, should I wash away the residue SDS from the gel slice so the band won't diffuse? Will the protein diffuse away from the gel if I don't wash away SDS? The SDS concentration is 2% in loading buffer and 0.1% in running buffer.
Thanks
And, another question, should I wash away the residue SDS from the gel slice so the band won't diffuse? Will the protein diffuse away from the gel if I don't wash away SDS? The SDS concentration is 2% in loading buffer and 0.1% in running buffer.
Thanks
when i send my protein for in gel digestion for mass spec, i usually send the gel peice in smal microfuge tube with or without 5% acetic acid
iam not sure if this will be fine for your method. but I never freeze my gels
u can rather keep a 4 degrees.
-mabusheh-