Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

strange expression model - (Jun/02/2005 )

one of the shorter protein i am expressing now was induced strangely. after two hours induction with IPTG i can see a quite strong band of expression, strangely after 4 hours induction the band become faint and even can not see it expressed. Does anyone have the same situation? really interesting. What is the protein going to ? mad.gif laugh.gif

-cathy-

Anyone has faced the same situation? just wondered? what is the possible reason?
Thanks million rolleyes.gif

-cathy-

I've never seen it, but could it be degradation? Or maybe aggregate into bigger molecules and shifted on the gel. Just guessing smile.gif

-xiaoyang12-