Biotinylation - (Oct/18/2005 )

Hi all,

I have a doubt regarding proteins biotinylation. As the kit I will use says, biotin reacts efficiently with primary amino groups. The question is if primary amines from both arginine and lysine aminoacids will react with biotin. And if not, why not.

Thanks in advance

Celvas

As I know, primary amines are the most reactive. Lys which has primary amine on its side chain is fairly abundant in the proteins. Reactivity with the secondary amines (arg), sulfhydryls (cys) is often negligible in the presence of primary amine grops. Tertiary amines are not reactive.

Ok Alesia, thank you very much for your response
But as I know arginine has both secondary and primary amines in its side chain, has not it?
Or these amine groups are both in electronic resonance therefore coexisting as both primary and secondary amines?