native gel -- protein migration - (Mar/10/2006 )

HI,

I just wondering that if my protien PI is 9.0, and I run it in conventional native acrylamide gel (stacking pH = 6.8, resolving pH = 8.8) using Tris-HCL/Gly buffer (pH = ~8.5), will the protein be able to enter the gel? Because under such condition my protein should have a positive net charge...

Thanks a lot:)