Extinction coefficient - (Jun/26/2006 )
Hi all,
I am working with papain and wanted to know about its extinction coefficient at 280 nm. I searched the internet and found it to be 25 but when I checked through the ProtParam tool of expassy it showed me the value as 56185.
Does anyone know what the exact value of extinction coefficient is for papain.
Regards,
Ansh
-Anshul-
what are the units of your 2 values?
-fred_33-
QUOTE (fred_33 @ Jun 27 2006, 11:11 PM)
what are the units of your 2 values?
The values given on the internet did not have any units. I Checked it by calculating the extinction coefficient on the basis of number of Trp, Tyr and Cys residues and it also gives me a value of 56185 M-1 cm-1. So i think this one should be correct.
Thanks for the reply
-Anshul-