Membrane protein extraction - What about MEM-PER kit (Pierce)? (Aug/16/2006 )
Hi,
I would like to extract membrane protein. I can't use ultracentrifugation. I would like to know if someone has already used the "MEM-PER Eukaryotic Membrane protein extraction reagent kit"? Does it work well or not?
Thanks in advance.
-vvmice-
QUOTE (vvmice @ Aug 16 2006, 04:37 AM)
Hi,
I would like to extract membrane protein. I can't use ultracentrifugation. I would like to know if someone has already used the "MEM-PER Eukaryotic Membrane protein extraction reagent kit"? Does it work well or not?
Thanks in advance.
I would like to extract membrane protein. I can't use ultracentrifugation. I would like to know if someone has already used the "MEM-PER Eukaryotic Membrane protein extraction reagent kit"? Does it work well or not?
Thanks in advance.

It works quite well. Follow the Pierce protocol carefully and it will work.
-KF_Path-