Can we freeze mitochondria after isolation? - (Aug/10/2006 )

Hi!

Can anybody tell me if we can freez mitochondria after isolation????
I want to do immunostaining on isolated mitochondria next day, so will they be viable next day or not??? though viability is not needed but I want them to stick to the coverslip.
and what if I want to do functional studies on these mitos???

thanks in advance for suggestions and explanation.

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Functional studies shouldnt b a problem after freeze thaw except u wil lose a bit of activity. U have to calculate that for urself.

For immunostaining, just fix them and then leave them in PBS-4C and work on it when u have time.

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thanks a lot!!!! I really appreciate your comments.
For fixing, do they need to be in suspension..... I generally put a drop on coverslip, and in 15-20 minutes they stick to it and then I do fixing/immunostaining.
After isolation, I place them on dry ice in a tube... and plan to do fixing and immunostaining next day after thawing. I guess it will work if what you said is right. what you say???

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It should work for immuno staining.

Good Luck !!!

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