Anti-digoxigenin Immunoprecipitation of Proteins

Has anybody performed this type of immunoprecipitation before? Is it possible, and if so, could I have a protocol for it? I've seen a few articles mentioning this technique online, so I believe that it is possible. However, I wanted to find somebody who's actually done it successfully.

Thanks,

Nathan

QUOTE (lomei @ Nov 14 2006, 10:40 PM)

Has anybody performed this type of immunoprecipitation before? Is it possible, and if so, could I have a protocol for it? I've seen a few articles mentioning this technique online, so I believe that it is possible. However, I wanted to find somebody who's actually done it successfully.

Thanks,

Nathan

please be more precise; which protocol do you mean?

QUOTE (kosmodrom @ Nov 15 2006, 03:23 AM)

QUOTE (lomei @ Nov 14 2006, 10:40 PM)

Has anybody performed this type of immunoprecipitation before? Is it possible, and if so, could I have a protocol for it? I've seen a few articles mentioning this technique online, so I believe that it is possible. However, I wanted to find somebody who's actually done it successfully.

Thanks,

Nathan

please be more precise; which protocol do you mean?

I'm sorry. Specifically, I mean immunoprecipitation of a Digoxigenin-labelled protein using an anti-digoxigenin antibody. Thanks everybody.

Anti-digoxigenin Immunoprecipitation of Proteins - (Nov/14/2006 )