Tricine gel storage - can i store fully made gels overnight? (May/02/2007 )
Hello everyone!
I have a nice simple question for you all,
If I make Tricine mini gels. can I store them overnight before I use them?
I have always been told that with the Laemmli gels, you shouldnt pour the stack gel onto the resolving gel to leave overnight because of the difference in pH. (can anyone explain further?)
I have just read in a pinned post in immunlogy that you should leave gels overnight to ensure they are fully polymerised. Would this mean just the resolving gel? or all together?
With the Tricine gels, can you store overnight and if so, does it matter if you make the stack as well, because there is no difference in pH between the two parts, as the same buffer is used for both.
Any help would be much appreciated!
[quote name='Flour Power' date='May 2 2007, 04:33 AM' post='96282']
Hello everyone!
I have a nice simple question for you all,
If I make Tricine mini gels. can I store them overnight before I use them?
I have always been told that with the Laemmli gels, you shouldnt pour the stack gel onto the resolving gel to leave overnight because of the difference in pH. (can anyone explain further?)
I have just read in a pinned post in immunlogy that you should leave gels overnight to ensure they are fully polymerised. Would this mean just the resolving gel? or all together?
With the Tricine gels, can you store overnight and if so, does it matter if you make the stack as well, because there is no difference in pH between the two parts, as the same buffer is used for both.
Any help would be much appreciated!
Don't worry! Prepare your Tricine minigels and store them overnight without any problems, only cover your gel with wet paper to prevent drying stack gel.
ABOUT reply: I have always been told that with the Laemmli gels, you shouldnt pour the stack gel onto the resolving gel to leave overnight because of the difference in pH. (can anyone explain further?)
Biorad provides minigels with polymerised stack and resolved gels ( ready to use) . It is commercial product and no problems with PAAG occur.
ABOUT Reply: I have just read in a pinned post in immunlogy that you should leave gels overnight to ensure they are fully polymerised.
It is enough to leave at 1 hour at RT for proper polymerisation. Sometimes we add 4-6 fold less PSA and TEMED and leave for tonight at RT to obtain more regulary polymerised gels to improve resolution.
I have a nice simple question for you all,
If I make Tricine mini gels. can I store them overnight before I use them?
I have always been told that with the Laemmli gels, you shouldnt pour the stack gel onto the resolving gel to leave overnight because of the difference in pH. (can anyone explain further?)
I have just read in a pinned post in immunlogy that you should leave gels overnight to ensure they are fully polymerised. Would this mean just the resolving gel? or all together?
With the Tricine gels, can you store overnight and if so, does it matter if you make the stack as well, because there is no difference in pH between the two parts, as the same buffer is used for both.
Any help would be much appreciated!
I never heard aboute these problems. We, and a lot of collaborating labs, store the TRIS-Glycin gels, with stacking gel ontop, for several days. Maybe there is a little intermix between both parts of the gel, but we never ever saw problems. "Fully polymerised", hm, never care about that. I need a page now, I use the fresh gel now...
Great, thanks both of you,
so you store at 4oC in wet tissue? or is cling film okay or something?
so you store at 4oC in wet tissue? or is cling film okay or something?
Well it's enough!
It wouldnt really dry that much even though you just leave it overnight. But of course wet tissue will do just fine.
we use wet paper towels around the edges and plastic wrap around the whole mess.