transfection - dna chloroform (Jan/19/2007 )
have some general doubts,
what will happen if we treat DNA with chloroform. I found a drop in transfection with the samples treated with chloroform.Is there any chemical interaction occuring here?
-mini-
QUOTE (mini @ Jan 19 2007, 01:23 AM)
have some general doubts,
what will happen if we treat DNA with chloroform. I found a drop in transfection with the samples treated with chloroform.Is there any chemical interaction occuring here?
what will happen if we treat DNA with chloroform. I found a drop in transfection with the samples treated with chloroform.Is there any chemical interaction occuring here?
Be sure that you dont lose any DNA during your extraction process. Other than that, I dont see areason why would cause a drop in transfection efficiency.
-genehunter-1-