3' bioninylated oligos for EMSA - (Jan/19/2007 )
I'm going to perform non-radioactive EMSA using Pierce's Lightshift kit, so I need 3' end-labeled oligos. I'm going to use 7 different probes, so I need 14 different oligos. I used oligos from Eurogentech and they work OK, but I was thinking about looking for cheaper suppliers, because 14 oligos mean a lot of money (more than 2000 €). Another possibility would be using Pierce's Biotin 3' end DNA labeling kit (20 reactions), but I heard that troubleshooting could be long so ordering custom oligos would be the more cost-effective way.
What's your opinion? Thanks in advance!
Felip.
-drfelip-
QUOTE (drfelip @ Jan 19 2007, 04:21 AM)
I'm going to perform non-radioactive EMSA using Pierce's Lightshift kit, so I need 3' end-labeled oligos. I'm going to use 7 different probes, so I need 14 different oligos. I used oligos from Eurogentech and they work OK, but I was thinking about looking for cheaper suppliers, because 14 oligos mean a lot of money (more than 2000 €). Another possibility would be using Pierce's Biotin 3' end DNA labeling kit (20 reactions), but I heard that troubleshooting could be long so ordering custom oligos would be the more cost-effective way.
What's your opinion? Thanks in advance!
Felip.
What's your opinion? Thanks in advance!
Felip.
Hi, I've used Pierce's Biotin 3' end DNA labelling kit and it was really easy to use and worked very well.
-marteca-
I agree. the 3' labelling kit is a cakewalk to use, and you will save a lot of money
-aimikins-