RNA electrophoresis - different concentration of ethidium bromide between gel and buffer (Oct/14/2008 )

Hi!

I ve made by mistake a 1% agarose gel that contains a higher concentration of ethidium bromide
(around 1µg/ml) than the usual 0.5 µg/ml.
The buffer in the tank contains 0.5 µg/ml Ethbr. Can I still use this gel for RNA electrophoresis or should i make a new one?

thanks!!

You need not to worry. Ethidium bromide in running buffer is not necessary because you have stained on your gel.

If you ran denaturing RNA gel you would see ethidium bromide glowing in yellow instead of orange.

thanks a lot!!!