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Arg-F lac E. coli - (Oct/09/2008 )

does anyone know the exact mutation location in Arg-F-lacZYA mutant of E. coli such as DH5a? i want to know the nature of the mutation done. Im wondering if the lacI gene is still intact in those strains? Thank you very much

-arvinsign-

Probably not. Someone, somewhere may have sequenced that region, but if they have, it has not been reported. Do the world a favor, and PCR that region of DH5a, sequence it, and then deposit it in Genbank. The strain pre-dates routine sequeincing.

-phage434-