Large insert cloning - (Mar/01/2005 )

Hi everybody,
have any one of you done large insert cloning? I am talking about the isert size from 5-20kb!
Any help regarding protocols and ideas on what plasmids/bacmids could be used is greatly appreciated!!!

Thank you!

Dragan

This thread may help
http://www.protocol-online.org/forums/inde...?showtopic=3434