NAC (No Amplification Control) - (Jun/02/2005 )

Is that polymerase or reverse transcriptase that is omitted in NAC reaction?

Thanks.

-indoubt-

I would say no RT, as without RT you should not get any amplification unless there is contamination with gDNA!

Nick

-methylnick-

QUOTE (methylnick @ Jun 2 2005, 06:36 PM)

I would say no RT, as without RT you should not get any amplification unless there is contamination with gDNA!

Nick

so I don't add reverse transcriptase, but i have to add DNA polymerase?

-indoubt-