cloning primer orientation - orientation and complement of primers in a cloning (Jun/07/2005 )

So heres the problem:
I have PCR products that have been amplified with primers, we will call them A and B. I get that now my product looks in some way like:
----/---------------------
-A PCR PRODUCT
---------------------/------
PCR PRODUCT -B-
So thats crudely what I have right?see the pcr primer attachment

Now this product has been cloned into an E.coli cell via a plasmid. This plasmid has primers called M and N say so now what I have is for example:

---/------------------------/-----/----------------------/----------------/-----------/--------
N plasmid sequence A PCR PRODUCT reverse of B Plasmid
---------------
reverse M

Like that or the reverse depending on the prientation of the pcr product in the plasmid right? - see figure 2 for a better picture.

so now, i want to identify the orientation of the pcr product in the plasmid. so i have amplifed using N and B for example. does this mean that when i send this for sequencing with prmer N i will get back a sequence that has:
- N - Plasmid - A - PCR - reverse of B - plasmid - reverse M
or
- N - plasmid - reverse A - PCR - B - plasmid - reverse M
?????????????????? this may be better drawn in figure 3 attached.

I am rather confused and would appreciate someone making this clear to me.

THANKS

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hi
i can understand quite your problem, but may you clarify your figures??

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HI
i have added soem ratehr basic paint images that may help clarify my meaning.

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hi
according to your picture 2, if you're using primers n and B, the only way you can have PCR is - N - Plasmid - A - PCR - reverse of B - plasmid - reverse M

if you don't have PCR using N and B but you have one using N and a, that means you have - N - plasmid - reverse A - PCR - B - plasmid - reverse M

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