InFusion - (Jul/14/2008 )
Has anyone tried using exonuclease III to conduct a ligation free cloning with any luck instead of using the InFusion Kit?
-Kristinee-
QUOTE (Kristinee @ Jul 14 2008, 10:29 PM)
Has anyone tried using exonuclease III to conduct a ligation free cloning with any luck instead of using the InFusion Kit?
No, but this is a good paper from nature methods that covers a similar technique.
[attachment=4981:Li_and_E...LIC_2007.pdf]
-swanny-
QUOTE (swanny @ Jul 15 2008, 01:02 AM)
QUOTE (Kristinee @ Jul 14 2008, 10:29 PM)
Has anyone tried using exonuclease III to conduct a ligation free cloning with any luck instead of using the InFusion Kit?
No, but this is a good paper from nature methods that covers a similar technique.
[attachment=4981:Li_and_E...LIC_2007.pdf]
Thanks! -miss cell-