Annealing buffer for DNA oligos - RNA interference (Nov/21/2005 )

Hi all,
i need composition of suitable annealing buffer for siRNA DNA oligos.
waiting for your swift reply
regards
amit

Hi Amit,

I guess you are trying to construct shRNA using DNA oligos, right? Then you can find the buffer on this page
http://www.protocol-online.org/prot/Molecu...igos/index.html

hi
there was a dicsussion on this topic here