allelic variants in a population - genomic DNA that was used for Human genome project (Nov/19/2005 )

[font=Fixedsys]Hi all
Can anyone tell me about the genomic DNA that was used for Human genome project. Where was it isolated from , I mean what kind of cells...Was the genomic DNA used the same for all the experiments that were carried out?

If the genomic DNA was isolated from one particular cell what are the chances that the sequence that is given for the various sequences of DNAs and mRNAs in the NCBI database is the same for sequences derived from all of us....

basically I am trying to understand the meaning of the term "allelic variants in a population"
So if I have three mRNA transcript variants reported for a gene which only differ by 60-100 nucleotides how do i know if they are trancript variants or only "allelic variants in a population"
It is a very vague question..i know but please help me understand this if anybody can...thanks

[font=Lucida Console]

Hi,

you can find more information about the source of the DNA from 'the' human genome in the paper describing the first draft sequence (Lander et al., 2001, Nature, 409: 860-921). The majority of the human genome sequence is however primarily derived from one library (RP-11).
It's difficult to answer your question about the allelic variants not knowing the differences between your sequences. You should analyze the composition of your sequences, maybe they are the result of alternative splicing?

I hope this helps you,
Karl