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Housekeeping gene for RT-PCR on plant - RT PCR (Jan/31/2006 )

Hi everybody smile.gif

I want to know which of the house keeping gene in plants other than tubulin and 18r DNA can i use to standardise my RTPCR results? I want one with atleast an intron of 100-200bp size so that i can clearly distinguish amplification between gDNA and cDNA!

-vishy-

Hi,
Check out this paper

http://www.gene-quantification.de/brunner-hkg-2004.pdf

-vopsideisi-