Housekeeping gene for RT-PCR on plant - RT PCR (Jan/31/2006 )
Hi everybody
I want to know which of the house keeping gene in plants other than tubulin and 18r DNA can i use to standardise my RTPCR results? I want one with atleast an intron of 100-200bp size so that i can clearly distinguish amplification between gDNA and cDNA!
-vishy-
Hi,
Check out this paper
http://www.gene-quantification.de/brunner-hkg-2004.pdf
-vopsideisi-