BCA assay - folded or denatured protein (Jul/15/2005 )

Hi all

I run a BCA assay (bicinchoninic acid assay) to determine the concentration of protein in cell extracts.

Will this assay still work with denatured protein that has been heated in laemmli buffer.

i.e. does protein still reduce Cu(II) to Cu(I) regardless of whether it is folded or denatured

Confused!

Thanks in advance

since peptide bonds and AA-residues are still present BCA will be ok

http://www.sigmaaldrich.com/Area_of_Intere...c_Acid_Kit.html

suggestion: in this case reaction should be easier; is the signal enhanced?
Seb_

hi
assuming BCA liks to peptidic bond it would be ok.