experimental determination of protein function - (May/15/2008 )

Hi there:

I would like to know what type of experimnts can i use for determining the function of my protein?
My protein is complemnt facor B (CFB) involved in the alternative pathway of complement system to destroy pathogen.

Is the two yeast hybrid system can be used for determination of CFB function. Am i right in my thought.
Is there any alternative experiment to determine CFB function?

Thanx

Here is a short list:

1. Over-expression in some cell lines (protein microinjection, protein transduction, transient or stable transfection of expression vector)
2. Knock-down (siRNA, inhibitor compound)
3 Above followed by expression microarray, proteomics (2D, protein array)
4. Mutation analysis (truncation, AA modification)
5. Knockout and transgenic mice (or some other organism) for your gene.
6. Interaction analysis may give clue to protein function, if the binding partner is famous (Y2H, Co-Ip, MS/MS and two dozen others)
7. bioinformatics for domains, post-translational modifications etc.
8. Any functional assays available in your system (example: clotting time, cytokine induction etc)



...