Recombinant protein stability - stability issue (Jun/04/2008 )
I have a recombinant protein thats 115aa long. The protein is not stable. after 10 days it loses about 15% activity and just keeps dropping. by 14 days about 40% of activity is lost. I want to stabalize this so attain at a minimum 28 days stability. Please assist with additives that may aid in stabalization (PBS buffer with protein and tween etc...)
-navadann-
QUOTE (navadann @ Jun 4 2008, 04:56 AM)
I have a recombinant protein thats 115aa long. The protein is not stable. after 10 days it loses about 15% activity and just keeps dropping. by 14 days about 40% of activity is lost. I want to stabalize this so attain at a minimum 28 days stability. Please assist with additives that may aid in stabalization (PBS buffer with protein and tween etc...)
These links will give you a lot of tips on protein stabilization:
protocols - http://search.vadlo.com/b/q?sn=158621799&a...ation&rel=0
Lectures - http://search.vadlo.com/b/q?sn=158621799&a...ility&rel=2
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