Tris Tricine Gel Running Buffer - (Sep/27/2007 )

Hello. I was wondering if anyone has a recipe for the running buffer for a Tris Tricine gel where I can run both chambers in the same buffer. Our gel apparatus leaks and I think the anode and cathode buffers suggested by Schagger and VonJagow will mix. I know that some companies sell one buffer to run these gels in, does anyone know the components of this buffer? Thanks so much.

fix the leak.

if it is a gasket then add vacuum grease to seal the plate to the apparatus.

if it is the apparatus (ie-cracked plastic) then fuse it with methylene chloride.

run the gels the way they were intended to be run.

that being said, invitrogen sells novex tricine-sds running buffer.