dUTP s - Explanation ! (Jan/24/2007 )

Hello !!!

Could anybody please tell me, what is a dUTP ? What does that mean ????

Im about to do a TUNEL and dont understand what is a dUTP....

Thanks !!!

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a dUTP is a Nucleotide (2’-deoxyuridine 5’-triphosphate) like A, T, C or G!
The Tunel-methode idientifies apoptotic cells by using terminal deoxynucleotide transferase to transfer biotin-labled dUTP to strand breaks of cleaved DNA (occuring in apoptotic cells wherin DNA is degraded by endonucleases). These cleavage sites are then detected by horseradish-peroxidase conjugated to streptavidine (binds to biotine with high affinity). HRP converts DAB (Diaminobenzidine) to a brown colour.
Thats what i know!
Best wishes!

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