whole gene synthesis for expression - (May/02/2007 )

Hi

I cloned a gene in several vectors and look for expression but none of the cases yield the protein. Then i switched to yeast expression but the result was same no expression. Now i am thinking to synthesize the whole gene by optimizing the codon for expression in bacteria by artificial gene synthesis. Had there been anybody done this previously to overcome the protein expression problem. I wanted to make sure that this strategy would work because the syntheis will cost very high. Please give me details of the following

1. How to optimize the codon for expression in E coli? Any software available
2. Which gene syntheis company is good for the synthesis?

Hello sktewary,

This is not an answer to your question, you say you get no expression. How come, is it leathal? wrong folding / lack of activity? I would think, ad hoc, that it should be possible to express it some how, without doing synthesis. Cheers