DNA molar calculation - (Jul/06/2008 )

Hi
Anybody knows how to convert the nanogram of DNA into femto mole of DNA.

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It's a simple calculation with n (moles) = mass (g) / molecular weight (MW) but the tricky bit is knowing the molecular weight of your DNA. Rest assured though, you don't need to calculate it manually (imagine that), rather use calculators such has the one below and the tough part is done for you:

http://www.changbioscience.com/genetics/mw.html

Good luck, Rob

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Those websites are always very useful; however doing it manually is far from difficult. All you need to know is the size of your DNA and then multiply the no. of bp by 660 (1 bp ~ 660 Da), and there you have the MW of your fragment. This way you know the HOW and WHY, and then you can keep using the website but understand the rationale behind.
Sorry if it sounded pedantic or patronising, far from the intention.

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biomath claculator from promega do it well and gives you a clear equation to do such operation.

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