PCR Performance Test - QC test for new Taq Polymerase (Jun/25/2008 )

Hello there,

I really need help on this topic. I have a new develop recombinant DNA taq polymerase....my issue here would be

1) how do i go about to test on the performance of the DNA amplification? from what i could find in the internet, PCR performance test usually been done on lambda DNA and human genome DNA as the template.

2) can someone teach me how to perform or how to detect the endonuclease and exonuclease activity of the enzyme (Taq polymerase)?do you have any protocol?

Please, please someone help me with these issues

Thank you in advance

-dianben in need-

thank you cellcounter. I will try look into this channel.Do you have experinec on this?

hallo, to tst polymerase you suppose to use 10 fold dilutions of template
short the time for the PCR steps and you will see how strong is or quick is polymerase
and amplify known sequence clone it and sequence so you can see the fidelity
use hard substrate as cell lysat for amlification
....
good luck