RNA precipitation with DNase treatment - (Jun/19/2008 )
Hi!
I have to concentrate my RNA samples for real time PCR. Have tried with Qiagen kits but the concentrations are too low (in what comes out). So I will now precipitate the RNA with salt and EtOH. My problem is that I want to DNase treat the samples since I will use them in q-pcr reactions. Does anyone have any suggestions how to do this? Can I just include it somewhere in my precipitation?
Thanx a lot!!
Zyrlex
-zyrlex-
QUOTE (zyrlex @ Jun 19 2008, 06:59 AM)
Hi!
I have to concentrate my RNA samples for real time PCR. Have tried with Qiagen kits but the concentrations are too low (in what comes out). So I will now precipitate the RNA with salt and EtOH. My problem is that I want to DNase treat the samples since I will use them in q-pcr reactions. Does anyone have any suggestions how to do this? Can I just include it somewhere in my precipitation?
Thanx a lot!!
Zyrlex
I have to concentrate my RNA samples for real time PCR. Have tried with Qiagen kits but the concentrations are too low (in what comes out). So I will now precipitate the RNA with salt and EtOH. My problem is that I want to DNase treat the samples since I will use them in q-pcr reactions. Does anyone have any suggestions how to do this? Can I just include it somewhere in my precipitation?
Thanx a lot!!
Zyrlex
You can first treat your RNA with DNase, inactivate it, and then precipitate the RNA. Alternatively, and less preferably, you can ppt your RNA, followed by DNase Turbo treatment prior to qpcr.
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