PCR off of Genomic DNA problems! - -why is my gene without introns showing up?- (Apr/18/2005 )

I think the title pretty much says it all.

I did genomic DNA extraction
Amplified with PCR

When i ran it on a gel i can see very clearly that my gene without introns is ALSO present along with my gene WITH introns.

Anyone have any ideas why this is so?

Am i forgetting some basic genetics and need to be reminded? haha...

Any response is appreciated!!

Thanks,

~QG

-QueensGal-

QUOTE (QueensGal @ Apr 18 2005, 12:45 PM)

When i ran it on a gel i can see very clearly that my gene without introns is ALSO present along with my gene WITH introns.

Anyone have any ideas why this is so?

could it be a pseudogene within the genome. ie: a copy of the gene but in an inactive form?

Nick

-methylnick-

Hi,

Sorry, I could not get it. How did you come to the conclusion about the identity of the extra band in your gel? Did you sequence it to verify?

SV

-SVTX-

hi
but how do u know its the gene without introns, if you work with genomic DNA? couldn't it be some nonspecific amplification
chear
chandima

-chandima-