Asymmetric PCR - (Mar/06/2006 )

Does any one know that why it is difficult to obtain reproducible result in asymmetric PCR each time?

The concentrations of the primers have to be perfect or else:

a) you obtain only dsDNA (too high a concentration of both primers)

you obtain no product

-Matt

thanx for ur reply.
But i kept the everything same, i mean the ration of concentration of both primers, cycle programme and the amount od DNA used. But the results were not reproducile?


-Matt
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