electrophoresis Methods, Protocols and Troubleshootings

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Electrophoresis Related Discussions

PCR product appear two close band in my gel! - (reply: 3)
230nm peak with NanoDrop - after gel purification (reply: 2)
Running Simple Gels w Bacterial DNA - (reply: 4)
reason for the browning of the silver gel - (reply: 1)
Weird migration on gel - (reply: 19)
Concentrating Conditioned Media for use on a non-denaturing agarose gel - (reply: 2)
Renaturing SDS Gel? - (reply: 1)
SDS gel - (reply: 1)
microsatellite (SSR): Agarose gel vs polyacrylamide gel. - (reply: 1)
2d electrophoresis with carrier ampholytes - 2d electrophoresis with carrier ampholytes (reply: 2)
TAE gel in TBE buffer - Does it work? (reply: 6)
gel image analysis software - (reply: 2)
How should products of first-strand cDNA synthesis look like on a gel? - (reply: 4)
how to analyze a gel image - (reply: 1)
Samples not on a straight line caused by gel dryness? - (reply: 4)
Uneven SYBR green staining/quantitative DNA gels - (reply: 1)
denaturing gel for RNA - (reply: 6)
running a gel on a colony - basic question (reply: 2)
expanded band of the purified protein on silverstain gel (even in low expression - (reply: 6)
RNA gel - interesting bands? some help - (reply: 3)
RNA gel - very interesting bands - (reply: 1)
RNA Electrophoresis Hints - (reply: 2)
any suggestions ragarding tricine gels - (reply: 3)
Problem with agarose gel electrophoresis - (reply: 2)
How do cellular fractions look like on SDS-PAGE gel? - what bands am I supposed to see in different fractions? (reply: 2)
protein spreading in gel - (reply: 1)
2D gelelectrophoresis: prestaining of proteins - (reply: 4)
Tris Hcl running gel buffer problems - (reply: 6)
Protein purification by two stage chromatography - MDH by anion exhange & gel filtration (reply: 1)
Gels waiting for 90 min before transfer? - (reply: 6)
Good DNA quantity with nanodrop but nothing on the gel - (reply: 8)
Gel band of bacteria genomic DNA - (reply: 1)
what do you think of this gel ? - (reply: 13)
band distortion in DNA gel electrophoresis - (reply: 1)
Agrose gel - (reply: 6)
protein gel electrophoresis - (reply: 7)
White precipitation in stacking gel - (reply: 10)
Protein Activity Assay With 2D Gel Spots - (reply: 1)
problems with electrophoresis run - (reply: 2)
High DNA Spec - Nothing on the Gel... - (reply: 2)
Help with Lab Math - preparing a gel (reply: 4)
plasmid isolation and gel - (reply: 1)
Is it possible to view mRNA on gel - (reply: 9)
Pulse Field Gel Electrophoresis (PFGE) - Secondary Restriction Enzyme Problem (reply: 2)
RNA electrophoresis - different concentration of ethidium bromide between gel and buffer (reply: 2)
My SDS-PAGE loading gets orange during the electrophoresis - (reply: 3)
How to make gradient gel for western blot ? - (reply: 8)
TBE gel, in TAE buffer. - (reply: 2)
SDS Gel for 2D -DIGE - Problems having SDS Page to polymerize (reply: 3)
how to distinguish supercoiled DNA from linear one in agarose gel - (reply: 2)
slow electrophoresis, somebody can help me? - troubles in electrophoresis (reply: 4)
Capture of silver stained gels - (reply: 1)
Agrose gel - (reply: 4)
Direct immunodetection within SDS-PAGE GEl? - Is it possible? (reply: 5)
SDS in gel or not? - (reply: 2)
How to rehydrate a SDS-PAGE gel - (reply: 2)
DNA Gel - (reply: 7)
Protein size on SDS gel - (reply: 1)
Repairing wire in gel apparatus - (reply: 3)
can I autoclave agarose? - (reply: 3)
Difference between TBE- and Denaturing Agarose Gel Electrophoresis of RNA - Gel for RNA (reply: 2)
Staining phosphoproteins on the SDS gel? - Can Pro-Q Diamond be used for a crude sample? (reply: 1)
Electrophoresis RNA - (reply: 1)
3.5% acrylamide gels and transfer - I need tips (reply: 4)
This is the gel pic that is confusing me and my boss - (reply: 7)
Stacking gel necessary? - (reply: 5)
gold view and sybr safe gel stain - any used gold view gel stain (reply: 2)
Silver Stain 2D gels - Trouble with silver staining 2D gels (reply: 1)
Gel extraction kit...which one is the best? Cheapest? Coolest? lol - (reply: 4)
Sterilization technique for gel - (reply: 3)
Non-denaturing western blot - I need a transfer protocol for non-denaturing gel (reply: 1)
Gel filtration - problem! (reply: 11)
MASCOT results vs 2D gel image : Which one do you trust? - Protein positive hits in MASCOT do not agree with a spot's position!! (reply: 2)
Improved electrophoresis of nucleic acids. - (reply: 15)
gel purifications of DNA fragm > 10Kb - (reply: 9)
Transformants pictures in gel - (reply: 2)
oxidized vs native protein? diffeerence expected on Isoelectric focusing gel? - why? (reply: 2)
Problems with zymography - Running SDS page gels protein wont migrate down the gel (reply: 4)
plasmid digestion gel - (reply: 4)
agarose gel electrophoresis - Question (reply: 3)
how much agarose to use - (reply: 5)
Extraction of PCR product from gel - (reply: 4)
gel and monoclonal antibody issues - (reply: 3)
How to extract Large protein from Low percentage SDS-Glycine-PAGE gel? - (reply: 1)
Native PAGE gel - (reply: 3)
Can we store the gel slice after cutting out the band and before gel extraction? - (reply: 3)
10% acrylamide/bisacrylamide, or 15% ? - SDS PAGE gel making (reply: 2)
Gel doc camera - Resolution (reply: 6)
2D gels.. comparison software? - (reply: 1)
2D Electrophoresis for protein inclusion bodies/lysate? - sample preparation? (reply: 1)
DNA gel electrophoresis problem? - (reply: 5)
DNA gel to Test Ligation? - (reply: 2)
Problem with drying down polyacrylamide gel - (reply: 4)
Frozen Protein A agarose beads - (reply: 1)
what could cause gel not to polymerize (solidify)? - gel making (reply: 8)
Need to sequence gel purified vectors exposed to UV? - (reply: 2)
poor electrophoretic protein transfer - (reply: 5)
RNA gel loading buffer? - (reply: 5)
Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
Sample (protein) recovery on 2D gel - (reply: 1)
multiple gel extractions - (reply: 5)
GEL Extraction (any advanced techniques ?) - (reply: 7)
Problems with bubbles in collagen gels - (reply: 1)
DNA gel problem - (reply: 13)
combining the results from different gels in western blot - (reply: 3)
Is it possible to use a PCR cleaning kit to clean a band from a TAE-Agarose gel? - (reply: 1)
transfering proteins from 2-D gel to nitrocellulose membrane - (reply: 5)
Great signal but nothing on gel - (reply: 4)
Dna purification from gel - (reply: 20)
Normal agarose gel vs formaldehyde agarose gel - (reply: 1)
New to RNA... Does this cDNA gel look OK? - (reply: 2)
Screening Tranformation Colonies: Funny Gel Pic and no plasmid?!?! - (reply: 10)
no high molecular weight bands seen.. know to be present.. - where did they go.. in a gradeint gel? (reply: 3)
2-D gel analysis - (reply: 3)
Homebrew Gel Documentation System - Burnout of Digital Camera? - (reply: 9)
Strange large bands on my PCR gel, has anyone seen this before? - PCR (reply: 3)
gel extraction - (reply: 1)
Can gel filtration effect buffer composition? - (reply: 4)
Plasmid DNA preparation - two DNA bands in gel (reply: 5)
Help troubleshoot SDS gel - smear down well - (reply: 2)
High concentration DNA on spectrophotometer but no band on agarose gel. WHere is - DNA from bone and teeth (reply: 5)
Coomassie Dye - SDS-PAGE gel - (reply: 2)
How to make a neat gel for master thesis? - Expressed protein on SDS-PAGe gel (reply: 3)
Proper ratio of stacking and separating gel - (reply: 3)
RNA denatruring agarose gel electrophoresis- help on sample preperation - (reply: 4)
benefits of urea in SDS PAGE gel - (reply: 9)
lowest concentration of RNA - to be visible on gel (reply: 3)
dna ladder seperation in gel - (reply: 4)
western blot -- running the gel - (reply: 6)
using piece agarose band directly on PCR - does it work? - (reply: 3)
what is more important in protein electrophoresis? voltage or current? - sds-page (reply: 10)
Silica Gel Chromatography beginners questions - Need some help getting started... (reply: 1)
Please help me with my sonication (gel included)... - I know, everyone asks the same thing, but I'm desperate (reply: 20)
Help! low molecule weight peptides electrophoresis! - (reply: 2)
How much RNA at least on agarose gel? - (reply: 3)
Native PAGE problem - Protein doesn't enter in resolving gel (reply: 1)
DNA spin column problems - Possible protein contamination and blank gel (reply: 6)
Denaturing Gels and non denaturing gels (Gel electrophoresis) - Gel electrophoresis (reply: 2)
Looking for perfect acrylamide gel EtBr stain - (reply: 4)
Native gels for proteasome activity assay - (reply: 3)
Gel destaining - (reply: 1)
no bands, no ladders, nothing at all in my gels - (reply: 7)
silly question...maximum loading for 1.5mm gel - (reply: 9)
reuse of mineral oil in 2 D gel electrophoresis - can we reuse mineral oil for 2 D gel electrophoresis (reply: 1)
Affect of pI on protein migration on SDS-PAGE gel - (reply: 5)
PGEM-T easy gel - partial digestion? - (reply: 2)
Running different % acyrlamide gels - (reply: 3)
Gel purification of large plasmids for PCR or sequencing, HELP! - (reply: 3)
running a 6% SDS gel - (reply: 1)
Removing bubbles from collagen gel - (reply: 2)
Polyacrylamide gels shriveling up during transfer - (reply: 1)
gel percentage & loading control for ATM and ATR? - western blotting (reply: 6)
linearized vector purification from TAE agarose gel - (reply: 5)
EC invasion into collagen gels and serum concentration - (reply: 3)
Western blotting conditioned media problem - Media will not separate when run on gel (reply: 2)
Current for gel electrophoresis - Fried my DNA I think (reply: 8)
How to improve the agarose gel? - (reply: 10)
problems with zymography gels - (reply: 1)
Use of silver staining for DNA in polyacrylamide gels... - or any other helpful suggestios to improve detection! (reply: 5)
Gravity flow gel filtration chromatography - (reply: 2)
Sephadex G-50 gel filtration chromatography - (reply: 6)
Sephadex G-50 gel filtration chromatography - (reply: 1)
native gel problem - (reply: 7)
SDS Gel - My protein bands go to the bottom of gel (reply: 9)
digested run slower on gel? - (reply: 6)
MoBio Plasmid Purification and Gel Extraction Kits - Problems with MoBio Kits (reply: 3)
2 - DE Gel - Protein Transfer - (reply: 2)
DNA Footprinting Gel - (reply: 3)
pulsed field gel electrophoresis of genomic DNA - (reply: 1)
Taking Agarose Gel Pic with Regular Digital Camera - (reply: 14)
Agarose gel - (reply: 6)
10kDa-20kDa proteins are missing on coomassie stained gel - (reply: 5)
sds page (gel preparation) - (reply: 4)
need help with my rna gel - (reply: 12)
Need help, what's wrong with my DNA gel? - (reply: 10)
gel bond and gel dryer - put gelbond in gel dyer (reply: 5)
Is there ay staining solution to stain colony on agarose gel - (reply: 2)
Ultra pure agarose - (reply: 4)
Question of QIAGEN's QIAquick Gel Extraction Kit - (reply: 10)
DNA gel purification without a spin column - (reply: 4)
DNA from Agarose gel basic questions - Basic functions of chemicals in DNA extraction from Agarose Gel (reply: 2)
Of voltage and bands - Agarose gel electrophoresis (reply: 3)
Genomic DNA smears on gel - (reply: 5)
RNA samples degraded in gel... where is the source of contamination? - (reply: 9)
basic dilution question for making gels - (reply: 1)
RNA gel - multiple bands - (reply: 2)
RNA Gels - (reply: 3)
Gel or column purify - (reply: 4)
Small DNA fragments, which electrophoresis shoudl i choose? - (reply: 10)
SOS: DNA purity problems - Low A260/230 of gel extracted DNA samples (reply: 4)
native gel problem: blurry bands - (reply: 11)
Native gel and salt - (reply: 2)
Qiagen gel extraction accident. - is all lost? (reply: 1)
Electrophoresis with sybrgreen - (reply: 5)
NuSieve GTG agarose gel takes forever to dissolve - (reply: 8)
What should 75 ug of total protein look like on gel? - Help - My 280 reading says I am loading 75 ug... (reply: 4)
Question about tricine gels - (reply: 1)
Tips on improving ligation+transformation from gel extraction - Let's contribute! (reply: 1)
gel compar II - (reply: 1)
Does phosphorylation affect protein migration in SDS-PAGE gel? - (reply: 9)
bumps in bottom part of sds gel - bumps in bottom part of sds gel (reply: 2)
Nano Drop measurements and RNa Gels incompatible? - (reply: 2)
protein-DNA interaction in gel filtration - (reply: 4)
gel ananlysis software - (reply: 6)
Agarose Gel instead of PAGE: Is it possible ? - (reply: 5)
gel formula - (reply: 1)
SDS gel image : why the band looks like 'stapler bullet' - (reply: 3)
Only 18S RNA Band shows up in RNA gel, why? - (reply: 2)
peptid conjugation to BSA ? No band in the gel? - (reply: 1)
Native acrylamid gel - (reply: 10)
Troubleshoot SB buffer electrophoresis problem - (reply: 6)
Protein A- agarose or protein A- sepharose? - immunoprecipitation (reply: 1)
Ratio of acrylamide to bis-acrylamide - different percentages for DNA and protein gels (reply: 1)
RNA gel extraction - (reply: 2)
PLasmid extraction: good nanodrop reading but NOTHING on the gel! - (reply: 4)
difference between Qiaex and Qiaquick gel extraction kits - (reply: 5)
RNA gel electrophoresis - multiple conformers or multiple products? - (reply: 1)
Cy3/Cy5 dyes and Urea denaturing PAGE gels - (reply: 1)
Gel extraction problem ~HELP~ - (reply: 3)
Acrylamide gels blurring DNA - (reply: 5)
High MW smear - how can I get all protein into the gel? - (reply: 3)
Does 20x TTE buffer REALLY expire? - polyacryamide gels; buffers (reply: 1)
plz guide me.i got many band in one well in agrose gel electrophoresis - PCR (reply: 8)
VERY SLOOOOOW running gels (SDS-PAGE) - (reply: 8)
Could you run Taqman PCR on gel to see the band? - (reply: 1)
extra bands on SDS PAGE gel - (reply: 4)
Gap between bands too small - DNA Gel Electrophoresis (reply: 6)
problems with RNA bands - on agarose gel (reply: 1)
problems with RNA bands - on agarose gel (reply: 2)
difference between "electrophoresis grade glycine" and regular old gly - (reply: 1)
Gel purification: SpinX vs commercial kits - Gel purification (reply: 3)
My DNA fragment in agarose gel is in 4 degree - Can I extract the fragment and use it for cloning??? (reply: 6)
Gel loading Question - (reply: 1)
Easy Question on seperating 1kb and 1.3kb gel bands - what percentage? (reply: 3)
electrophoresis for RNA - agarose gel or FA gel?? (reply: 9)
[western blotting] Loading control in 6% gel? - Please suggest good loading control protein of high molecular weight (reply: 2)
bands/dyes don't move on gel - (reply: 9)
shotgun cloning: gel shows inserts but only plasmid sequence obtained - (reply: 1)
Is there any good coomassie stain which is comparable to silver stain? - searching for a new staining method for 2D gels (reply: 6)
the problem in gel purification - (reply: 4)
How to choose the ready gel for western blotting - (reply: 3)
QIAEX II gel purification kit - (reply: 11)
RNA extraction/electrophoresis problem - (reply: 8)
need help, my collagen gels are watery - (reply: 1)
Why is resistance increasing with SDS PAGE gel run - (reply: 4)
polyacrylamide gel for DNA analysis - (reply: 4)
no band in 6% gel - (reply: 4)
Gel shift on formalin tissues? - EMSA (reply: 1)
Tris Tricine Gel Running Buffer - (reply: 1)
"Question"Why Gel Extraction Kit not working? - (reply: 6)
Freeze and crush method for gel extraction - (reply: 2)
Uneven band migration on agarose gel - (reply: 4)
band disappearance on agarose gel - (reply: 3)
TBE urea gel sample buffer - formamid or not? (reply: 2)
Formaldehyde Gel Electrophoresis on non-denatured/denatured RNA - No/Faint RNA bands after denatration (reply: 1)
Westerns from 3D collagen gels - (reply: 1)
recipe for SB buffer for electrophoresis - (reply: 4)
Gel without ethidium - (reply: 8)
sticky and blunt DNA on gel migration - (reply: 5)
Strange "V" band in Gel Shift - (reply: 4)
DNA yields with the Qiagen PCR gel purification kit - (reply: 8)
semi-dry transfer for midi gels - (reply: 4)
The top of PAGE gel is not polymerizing - (reply: 6)
gel elution problem - (reply: 1)
shaded bands in agarose gel - (reply: 8)
See small size difference on gel? - (reply: 2)
saving your gel for future - (reply: 3)
Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
2D Gel Electrophoresis Software - (reply: 1)
help with LDH separation using agarose gel electrophoresis - (reply: 5)
After extracting total RNA from tissue using trizol reagent and phase lock gel,h - I am going to extract bothe total RNA and genome DNA from one sample (reply: 4)
SDS Gel - (reply: 4)
White background on DNA gels - (reply: 8)
Circular ligation product migration on gel? - (reply: 1)
Setting tris-glycine gels and storing them BEFORE running them? - (reply: 7)
RNA gel electrophoresis - any recommendations? - (reply: 12)
Why do I got 3 bands of RNA gel? - (reply: 3)
question about agarose gel - (reply: 7)
Gel Fixation - (reply: 6)
glycerol [c] for dna in gel - (reply: 5)
Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
Bloting IEF gels onto PVDF membranes? - (reply: 1)
identifying a gel band by mass spectrometre? - problem in identifying the protein, no matches (reply: 13)
problem in separating gel - (reply: 6)
Gel purification of plasmid after each of these steps? - (reply: 1)
horizontal streaking in 2-D gel? - for help (reply: 4)
southern blot SSC bufffer doubt - do we need to use electrophoresis grade sodium citrate? (reply: 2)
Agarose gel solidifying - from asia-poland (reply: 6)
Lack of bands in SDS-PAGE gel - (reply: 4)
Gel Shifts: DIG labelling - (reply: 1)
DNA Extraction for Ladder Assay - Protocols to get best Agarose gels (reply: 4)
Electrophoresis problem - (reply: 9)
DGGE - PCR and gel conditions - (reply: 2)
SYBR Green Nucleic Acid Gel Stain - alternative for EtBr? (reply: 1)
Preparing Gel Loading Buffer - (reply: 6)
Agarose gel concentration for small and similar pcr pdts - (reply: 7)
fresh gel - (reply: 5)
do you subtract gel base intensity from PCR band intensity? - urgent require (reply: 3)
samples floating out of SDS-page gel well immediately after loading - (reply: 7)
Expect monomer on SDS PAGE gel get dimer or larger - (reply: 3)
How to dilute loading dye? (For gel electrophoresis) - (reply: 6)
Agarose 'rafts' blocking the top of sample well - Difficulty pouring a 3% gel (reply: 4)
Electrophoresis, pls urgent - (reply: 17)
Urea gels - (reply: 5)
Low concentration after gel purification - PCR product purification (reply: 6)
Free Agarose - Free Agarose (reply: 8)
Acrylamide gel with abnormal band - (reply: 4)
Elution of a smear from acrylamide gel - Electrophoresis and gel elution (reply: 2)
Two Tm peak in real-time but only one product on a gel? - What's goning on????? (reply: 4)
PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
Gel purification of DNA bands-any alternates? - (reply: 4)
LDH isoenzyme separation by Electrophoresis - (reply: 2)
Column chromatography with silica gel - (reply: 2)
real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
2D gels: Is there a protein assay thiourea is compatible with? - proteomics (reply: 2)
unsuccessful gel purification - (reply: 8)
gel purification for PCR product - (reply: 5)
Acrylamide gel preparation - for SDS-PAGE (reply: 4)
Help! I need to run collagen on SDS gel - (reply: 2)
sample preparation for 2d gel elctrophoresis - (reply: 2)
PRotein marker can give concentration of my protein in gel? - (reply: 4)
Gel appeared during serum collection - (reply: 1)
Gel shift with high isoelectric point protein - (reply: 1)
Quantifying gel purification products - (reply: 12)
capilary electrophoresis - (reply: 2)
strange red lines in all of my agarose gels from PCR - (reply: 2)
Ethidium bromide gel - (reply: 15)
About gel exlusion chromatography - (reply: 5)
electrophoresis buffer become very hot - (reply: 11)
protein extraction form SDS PAGE gels - (reply: 4)
Tricine gel storage - can i store fully made gels overnight? (reply: 6)
Need denature (94oc in 5 mintute) for agarose gel? - (reply: 7)
agarose gel electrophoresis - problems in marker migration (reply: 14)
Making high concentration agarose gels - A request for advice (reply: 7)
Differences between DGGE & standard electrophoresis? - (reply: 1)
Dissociation curve vs Gel image - Which one to trust? (reply: 1)
PAGE gel DNA band shape - (reply: 4)
Is 20% methanol sufficient to fix protein in gel? - (reply: 5)
DNA extraction from gel - (reply: 16)
electrophoresis question - (reply: 6)
Smearing antibody and DTT - how to interpret the gel? - Aggregated antibody (reply: 1)
help with agarose gel problem - (reply: 3)
How to quantitate the pcr product from gel picturess - (reply: 4)
Gel Loading- Equal Protein or equal volumes? - (reply: 2)
How to elute protein from SDS-PAGE gels - (reply: 3)
Electrophoresis buffer prep questions - (reply: 9)
How to run poly-lysine on tris-tricine gel - (reply: 4)
Extract DNA from gel - (reply: 5)
Pulse Field Gel Electrophoresis - for E.faecium (reply: 4)
Ligation reaction gel picture - (reply: 1)
Recipe needed for native gel - (reply: 1)
Transfection of expression vector for gel shift - how much vector should be transfected? (reply: 2)
What safety and risk assessments should i be aware of in doing PCR and gel elect - (reply: 2)
contaminated gel eluted DNA fragment - (reply: 8)
Improvement to Lammli Gel electrophoresis - The occasional gem (reply: 2)
Loss of phosphorylation after gel purification? - (reply: 3)
rehydrate dried gel - (reply: 4)
Uneven background of DNA electrophoresis image - (reply: 4)
i have to put gel until tomorrow - URGENT (reply: 6)
polyacrylamide gel electophoresis - (reply: 8)
melting a polyacrylamide gel? urgent - (reply: 6)
low melting agarose - low melting agarose (reply: 3)
Electrophoresis of DNA - (reply: 7)
Gel extraction protocol for DNA purification - Any suggestion? (reply: 11)
Locate a lab for PCR and Gel work - (reply: 2)
Protein high molecular weight standards for gel filtration - (reply: 2)
dna recovery from gel - (reply: 7)
moving of gel - from gel plate (reply: 4)
agarose gel melted after 4h running.. - (reply: 11)
Visualising 200-400+ kDa proteins by gel electrophoresis - (reply: 3)
dna on gel image help - (reply: 3)
PCR DNA IMAGES ON GEL HELP WANTED - (reply: 3)
FPLC: Gel Filtraito - Running Standards, Triton X-100, Mitochondria on a column (reply: 3)
I need your help regarding HAEMOGLOBIN ELECTROPHORESIS - (reply: 1)
Buffer BPTE for DNA electrophoresis - (reply: 3)
Serious Mini Gel Stack Problems. - (reply: 16)
can agarose be removed by phenol? - (reply: 5)
My DNA shoots out of the wells in my Agarose Gel... why? - Agarose Gel Loading Help (reply: 14)
2D protein gel for miRNA target screening? - (reply: 2)
running DNA on gel - (reply: 2)
low MW bands left on gel during transfer to PVDF - (reply: 1)
Agarose Gel Electrophoresis - Troubleshoot (reply: 12)
gel electrophoresis - problem with stacking (reply: 22)
large proteins don't analyse well-sds page gels - (reply: 12)
How long to store 1% agarose gels? - (reply: 11)
Simple Question... Volts/amps for running mini gels? - Which volts/amps are the best, mine do not work! (reply: 11)
Why high voltage is used in capillary electrophoresis? - (reply: 1)
making Ficoll gel loading buffer - (reply: 1)
What type of native gel (pH/acrylamide %) do I use for a protein with the pI=9.0 - (reply: 4)
extracting DNA frm agarose gel no result - (reply: 3)
Protein A agarose for CHIP--pre-treatment is needed? - (reply: 2)
How to take picture of dna electrophoresis without a gel documentation unit? - (reply: 3)
Blotting gel after Bio-Rad Coomassie staining? - (reply: 5)
Shadow in agarose gel - (reply: 8)
native gel? - want protocol (reply: 7)
HELP: Excision of gel band from EMSA gel - (reply: 1)
capillary electrophoresis - (reply: 1)
Help: ECM Extraction from cells grown in Alginate gels - (reply: 4)
Molecular weight determination by SDS page and gel filtration - (reply: 5)
DNA markers - Gel run agarose or PAGE (reply: 1)
Please check my ChIP sonication gel - (reply: 4)
destaining silver stained gels - (reply: 1)
Gel Electrophoresis & Staining - (reply: 2)
Help Reading Sonication Gels! - (reply: 4)
Frowns in polyacrylamide gel - (reply: 8)
useful tips for low melting temperature gel purification. - please share your experiences. (reply: 2)
high sizes on gel - (reply: 5)
running a gel with primers - (reply: 2)
Acrylamide/Agarose gels on nucleic acids... - Differential use...? (reply: 1)
ethanol precipitation before gel purification... - (reply: 5)
Questions about protein gel loading and immunoprecipitation - (reply: 4)
In gel electrophoresis, what are the effects of sample loading on band size? - (reply: 3)
What to load in empty well on SDS gel? - (reply: 8)
why protein gels are run at constant current and constant voltage? - (reply: 2)
Protein purification for rabbit injection - SDS-PAGE gel, excise band, inject into rabbit?? (reply: 5)
what speed to centrifuge down agarose bead? - (reply: 4)
proteomic study at 1-D and 2-D electrophoresis. - wants your opinion ! (reply: 5)
Uncut genomic DNA - Migrate through the gel (reply: 3)
analysis of DNA using BamH1 and agarose gel electrophoresis - interpretation of results! (reply: 5)
protein bands are not migrating over to the whole gels. - please suggest me something, very urgent ! (reply: 8)
Gel Electrophoresis Bowed Migration Pattern Troubleshooting - (reply: 3)
Problem with agarose gel? or with RNA ? - (reply: 7)
How can I dissolve 6%polyacrylamide gel? - (reply: 2)
In gel electrophoresis... - (reply: 2)
gel keeping for Mass Spec - (reply: 5)
What factors affect the separation and visualisation of DNA fragments in gel ele - (reply: 1)
can someone tell me about extraction DNA from polyacrylamide gel? - (reply: 10)
why not boiled before electrophoresis? - (reply: 9)
streaking in 2D gels - (reply: 2)
Does anyone add Etbr to running buffer used to make agarose gels? - (reply: 14)
Viral RNA - have you ever run it in a gel? (reply: 6)
Lab velocity - free samples for gels and UV cuvettes (reply: 5)
In DNA gel electrophoresis, what happens to lambda fragments after being treated - (reply: 2)
3D collagen matrice - Polymerization of collagen gel (reply: 5)
RT-PCR product dilution and electrophoresis - (reply: 2)
How to calculate DNA concentration after PCR manually - not using spectrophotometry or electrophoresis (reply: 6)
Qiagen gel extraction kit - strange buffer color (reply: 7)
Protein extraction from SDS gel - (reply: 6)
weak band during denature agarose gel electrophoresis of RNA - RNA denature agarose gel electrophoresis (reply: 3)
Low yield after gel extraction - (reply: 12)
Agaroase gel analysis of ligation reaction - (reply: 6)
Problem loading SDS-PAGE gel - (reply: 19)
Strange gel pattern, is EtBr the cause? - (reply: 1)
Help: Can't see any positive staining at all... SDS PAGE gel - (reply: 5)
basic gel question - (reply: 7)
Gel Shifts: Non-Specific Competition - (reply: 1)
advice on A/G agarose for ChIP - which gives the lowest background (reply: 3)
need advice on qPCR results (see attachment) - melting curve - gel picture (reply: 5)
Smear on gel after MiniPrep - Is this RNA? (reply: 10)
analysing gel bands - analysing gel bands (reply: 1)
RNA migration in agarose formaldehyde gel - (reply: 6)
Smeary DNA band on agarose gel - (reply: 5)
difference between RNA & cDNA gel image - (reply: 1)
Gel purification of PCR products - (reply: 2)
S-500 Gel filtration - molecular weight calibration (reply: 1)
how to run native PAGE gel? - (reply: 3)
Gel filtration estimations - (reply: 1)
SDS page gel GST beads or not to? - (reply: 3)
Expected bands in agarose gel electrophoresis - (reply: 3)
running itme for gels - (reply: 3)
HELP! PAGE Gel Running problem - (reply: 9)
DNA gel picture labeling - too many samples to label by hand - (reply: 2)
Native Gel bovine IgG - Native Gel (reply: 8)
Fuzzy bands on agarose gel after ligation - (reply: 7)
SDS-PAGE gel interpretation - sds page gel band reading (reply: 3)
phenol/CHCl3 vs purification from gel - (reply: 1)
PROTEIN AND DNA GEL ELECTROPHORESIS - GEL ELECTROPHORESIS (reply: 4)
protease bands on the gel.... - should they be visible? (reply: 11)
Single Colony Gels? - (reply: 7)
Gel and Ct-values relationship: is there a probe problem? - Bands on gel don't fit my expectations... (reply: 2)
Protein gel Loading troubles - Protein prep too viscous (reply: 4)
electrophoresis DNA yeast - (reply: 3)
Gel shift - (reply: 1)
differential electrophoretic migration of phosphorylated proteins - (reply: 2)
Screening for a 30bp difference using an agarose gel. - (reply: 4)
can protein A agarose precipitate anti goat IgG? - (reply: 2)
nucleic acids gel densitometry - (reply: 1)
DNA quantitation on agarose gel - (reply: 11)
Absence of bands in some lanes of gel electrophoresis - What are the reasons? (reply: 2)
Help: How to run Histone on SDS-PAGE gel? - (reply: 6)
RFLP - reading gels (reply: 1)
mutiple DNA bands on my agarose gel - (reply: 3)
RNA gel shift - (reply: 1)
smears on gel.. - REASONS (reply: 4)
gel purification of PCR product - (reply: 1)
which temperature should I run my 2. dimension gel at? - (reply: 3)
SDS-PAGE - CAN I USED 12% GEL FOR WHEAT LEAVE PROTEIN SDS-PAGE (reply: 1)
Etbr in agrose Gel - (reply: 5)
Metaphor agarose optimization - (reply: 1)
questions about native gel - (reply: 3)
Native Gel for QDs and Peptides - (reply: 8)
cannot see RNA on gel - (reply: 6)
Proteins samples not migrating from stacking into resolving gel - (reply: 6)
PAGE gel casting - (reply: 4)
Agarose gel for very small DNA fragments - (reply: 7)
Percentage of SDS- PAGE gel for co-IP - (reply: 1)
gel run - (reply: 1)
2D Gel electrophoresis analysis software - Is a good one free available? (reply: 1)
fading DNA bands on agarose gel - is it normal? - (reply: 4)
SDS-PAGE over heating and will western work from that gel? - sds page running and western blot of gel run under too much heating (reply: 9)
PCR band disappears in agarose gel! - Agarose gel electrophoresis (reply: 4)
Minimum sample required for visualization on gel - How to load 1ul of DNA on gel (reply: 3)
MOLECULAR TEST WITH !JUST PCR AND GEL! FOR A STARTER - PLEASE GIVE ME SUGGESTIONS (reply: 3)
A native PAGE - for a protein, 15.3 kDa, gel/run conditions? (reply: 2)
Elution from immunoprecipitation suitable for 2D-GE - Elution from immunoprecipitation suitable for 2D gel electrophoresis (reply: 1)
sds page - how to quantifing of gel doc (reply: 1)
DNA concentration too low for gel electrophorsis - (reply: 6)
Acrylamide gels: sample preparation with high salt concentration - (reply: 1)
How to store PAGE gel and keep the bands after gel is run? - (reply: 23)
Electrophoresis: only seperate DNA by size!? - (reply: 4)
Gel ELECTROPHORESIS ?PLEASE HELP? - DNA ISOLATION (reply: 1)
Should I clone my PCR product even though it is invisible on gel? - (reply: 5)
staining the gel - (reply: 5)
How to do gel filtration - (reply: 3)
Probe labelling foe gel shifts - (reply: 2)
Small peptides in gel - What to do to see a 2kDa peptide in coomassie gel (reply: 25)
Western blot gel running problem - Gel Electrophoresis (reply: 7)
UREA in SDS-PAGE - How to make the gel (reply: 1)
6% gel westren blot not working as well as 10% - (reply: 1)
TBE vs TAE for agarose gel electrophoresis - (reply: 36)
non-reductive gel to check the disulfide bond - (reply: 14)
Gel purified samples not sinking in gel well's - Not getting my samples to fall to bottom of well (reply: 8)
Why do we have to use MOPS and formaldehyde in RNA electrophoresis - (reply: 1)
Expired gel for WB - Can i still use it..(urgent) (reply: 4)
vertical agarose gel - (reply: 2)
A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
DNA purity by Agarose Gel Electrophoresis - (reply: 1)
plasmid DNA running on agarose gel to the opposite direction - (reply: 7)
problem with dna fragment extraction gel - (reply: 1)
biotinylated proteins purification from free biotin - dialysis vs gel filtration or what else (reply: 10)
Blue Native Gel Electrophoresis for membran protein complex - (reply: 1)
How do you store your gels? - (reply: 8)
Agarose gel for 580bp DNA - What are the suitable conditions? (reply: 8)
help with ligation and gel extraction - (reply: 5)
Problems with gel extraction and ligation - (reply: 12)
Why SDS page gel have to prepared freshly? - (reply: 6)
No PCR product detected in agraose gel - (reply: 7)
problem in my native gel - (reply: 4)
Does gel thickness affect light intensity of the DNA? - (reply: 3)
Native RNA gel - (reply: 1)
questions about the agarose bead protocol - (reply: 1)
Why can I not see bands on an agarose gel? - agarose gel visualization (reply: 5)
One specific DNA band became 2 bands after gel extraction? - (reply: 6)
using agarose for plates - (reply: 2)
no migration of DNA on electrophoresis - (reply: 7)
Problems in DNA sequencing after gel purification - (reply: 9)
Proteins and markers in top of gel - Running a gel (reply: 5)
Unwanted band seen along with my pcr product. - regarding PCR product with unwanted band formation during electrophoresis (reply: 3)
what is the meaning of de -gas the acrylamide gel mix? - (reply: 6)
SDS-PAGE gel preparation - (reply: 10)
sodium borate acid for DNA electrophoresis? - It's supposed to be much faster. Have you tried it? (reply: 13)
reuse PCR gel - (reply: 2)
which voltage for which agarose concentration? - assuming same size of gel, how should voltage be adapted to agarose concentratio (reply: 3)
Can we reuse agarose gel? - (reply: 10)
Question on Ethidium Bromide Gels - (reply: 2)
Allowed gel percentage to seperate proteins of 20kd or less - (reply: 1)
Gel percentage & cross-linker percentage, protein MW constrains. - (reply: 2)
Tris Tricine Gels for amyloid-beta Oligomers - (reply: 7)
extraction of DNA from agarose gels - (reply: 4)
Rhinovirus (RV) GEL FILTRATION - (reply: 1)
Storing gels . . . - (reply: 3)
protein diffusion in sds page gel - (reply: 2)
Streaks on 2D gels - (reply: 3)
to extract DNA from agarose gel - (reply: 11)
agar and agarose s - (reply: 4)
DNA agarose gels - trouble shoot weird gels (reply: 7)
eluting protein from the gel band... - (reply: 7)
Electrophoresis of Plasmid - (reply: 3)
Exposing southern gel to UV before transfer - (reply: 1)
Real Time: One Melting Curve 2 gel bands - (reply: 6)
Help needed-Protein extraction from native gel - (reply: 3)
gel shift assay help- big smear lane - (reply: 2)
Agarose gel electrophoresis - for separating bacteria cells?! (reply: 5)
formaldehyde in RNA gels - (reply: 4)
RNA gel troubleshooting - (reply: 8)
Strong distortion in bands of dna gel electrophoresis - (reply: 6)
Removal of etoh from purified samples - my gel purified samples contaminated with ethanol. how to get rid?? (reply: 6)
Unclear bands in bp marker of agarose gel - should i lower the voltage? (reply: 5)
Gel extraction - just wondering!!!! (reply: 8)
SDS-PAGE gel won't get solidified - (reply: 5)
Question about making low percent SDS-PAGE gels for big protein - (reply: 9)
Tricine SDS-PAGE gel under non-reducing - please tell me a protocol (reply: 1)
PFGE techinque - Pulse fiels gel electrphoresis (reply: 6)
Gel permeation & filtration - difference plz? (reply: 1)
Distinguish uncut vs. cut plasmids on agarose gel - (reply: 9)
FLAG M2 affinity gel agarose storage condition - (reply: 1)
protein contamination in DNA electrophoresis - (reply: 2)
protein elution from PAA gels - (reply: 2)
Problem in protein preparation in 2-D electrophoresis - (reply: 1)
Ramdom competitor in Gel Shift Assay - EMSA expert, please help! (reply: 1)
2-D electrophoresis problem... strange spots - (reply: 2)
Denaturing Agarose gel for RNA - (reply: 10)
SDS-PAGE % - what % gel should I use (reply: 5)
Problem pouring SDS-PAGE gel - (reply: 9)
Multiple bands on RNA gel, why? - (reply: 6)
VERTICAL DGGE GEL - (reply: 5)
need help with calcium phosphate gel - (reply: 5)
Different conformation of DNA after gel extraction? - (reply: 6)
protein tranfer from gel to membrane, reversible ? - (reply: 2)
Agarose gels and SB Buffer - (reply: 7)
different amount of template DNA added will give out different pattern in gel af - (reply: 4)
electrophoresis to resolve two DNA of similar size - (reply: 6)
Should I use LMT Agarose to purify genomic DNA? Help please! - (reply: 1)
Help me! No colonies! Problem with gel purification? - (reply: 5)
Protease: Post Electrophoretic Reactivation - (reply: 3)
loading protein onto SDS-PAGE gel - (reply: 2)
PCR doesn't work! - Falling in an empty gel "space" (reply: 2)
which gel type to choose for protein electrophoresis? - difference between types of gels (reply: 12)
Gel filtration - (reply: 3)
Developing Gel instead of PVDF - (reply: 3)
Qiagen Gel Extraction of PCR product - (reply: 7)
gels for RNA - (reply: 1)
Reagents used...in restriction digestion and electrophoresis - (reply: 2)
RFLP electrophoresis - (reply: 2)
native gel -- protein migration - (reply: 1)
Problem choosing the right kind of Precast gels - (reply: 1)
how much do you load on the gel? - do you really dilute to certain OD? (reply: 3)
denaturing gel - (reply: 4)
curved gel surface - (reply: 10)
lousy agarose gel - (reply: 2)
problem with RNA ladder in polyacrylamide gel - (reply: 1)
gel purification necessary for ligation? - (reply: 3)
purification of DNA extracted from agarose gel - does ethidium bromide remains / interefrence with ligation (reply: 1)
two bands after gel elusion instead of one - (reply: 6)
problem with gel filtration - (reply: 1)
multiplex analysis of amplicons on gel using fluorophores - what fluorophore/dye can I use (reply: 3)
Gel Extraction problems - using Invitrogen Purelink (reply: 1)
protein precipitation during gel loading - (reply: 3)
Wierd 1xTAE Gel - (reply: 3)
Gel shift assay for non purified protein preps - (reply: 1)
Having problem preparing sample for 2D gel. Help pls. - (reply: 2)
SDS gel strangely running: curved ladder - Ladder starts running vertical, and then levels horizontal in the running gel (reply: 7)
Agarose gel - DEPC-induced fragility? - RNA gels (reply: 2)
Please help with high-molecular-weight DNA gel electrophoresis! - (reply: 2)
can you ligate gel slices directly? - (reply: 8)
how to estimate amount of DNA using gel? - do u use agarose or acrylamide? (reply: 2)
DNA fragmentation- gel electrophoresis assay - molecular biology (reply: 1)
Low melt temp agarose not melting - (reply: 13)
Gel Filtration - which system is most suitable? - (reply: 3)
denaturating protein electrophoresis - (reply: 5)
DNA migrates faster the longer you run a gel? - (reply: 4)
gel purification of the vector... - is it good enough for ligation? (reply: 7)
decontamination of electrophoresis chambers... - what solution should be used? (reply: 2)
Gel loading buffer - (reply: 12)
Gel elution of a band - (reply: 4)
Crystal Clear Acrylamide Gels - Has anyone been able to cast an optically clear Acrylamide gel? (reply: 4)
Gel Filtration/Protein Precipitation - (reply: 1)
FISH probes - why check with gel (reply: 1)
DGGE gel problem - (reply: 1)
Drying SDS gels - (reply: 4)
Efficiency of the transfer in Western Blotting - How can you tell from the gel? (reply: 10)
transverse agarose pore gradient gel electrophoresis - anyobdy gotta protocol?? (reply: 2)
Qiagen Rneasy multiple bands in gel - (reply: 1)
Band diffusion of my PCR product in electrophoresis - (reply: 3)
extra band after gel extraction - (reply: 4)
Does BSA slow DNA migration in agarose gels? - (reply: 1)
Help with western blotting - protein not running in to seperating gel (reply: 1)
Verticals streaks in kinase assay gels - (reply: 2)
how do you determine the correct voltage and time of running of an agarose gel? - (reply: 6)
Tips on Casting acrylamide gel - (reply: 4)
Urea gel - preparation of urea gel (reply: 2)
Plused Field Gel Electrophoresis - (reply: 1)
tricin gel problem - shrinking of stacking gel (reply: 6)
Storing SDS-page gels - (reply: 1)
Extract hemoglobin from blood for starch gel electrophoresis - (reply: 1)
Differenciation between plasmid, super-coiled DNA and linear DNA - What is the difference between plasmid and other DNA on the gel? (reply: 1)
Best electrophoresis set up? Biorad vs. Hoeffer - (reply: 4)
Acrylamide gel to resolve DNA of similar size? - a little advise please (reply: 8)
Strange analytical gel for miniprep - (reply: 4)
Immunoblot (western) resolution vs electrophoresis resolution - (reply: 1)
Softwares and hardwares used for scanning gel electrophoresis banding pattern - (reply: 1)
electrophoresis of insulin - (reply: 2)
DNA purification from agarose gels - (reply: 2)
DNA Purification from gels - (reply: 4)
Hand-held UV wand: the most useful thing ever? - Gel electrophoresis, southerns and northerns (reply: 7)
How to analyze antigen-antobody complex on gel? - Which gel will NOT break the complex? (reply: 3)
clarification - plasmid and gel - (reply: 9)
RT-PCR- help - Polyacrylmide Gel electrophrosis for PCR products (reply: 3)
No DNA on gel, but get PCR product - (reply: 2)
Weird Band gel migration - Molecular biology (reply: 7)
RNA sample stuck in well during agarose electrophoresis - (reply: 1)
NATIVE gel band - sample running at the side... - My NATIVE gel run produced a band which has hollowed out central with visible st (reply: 1)
SDS-PAGE running with my sample widening? - my sample sort of grew fat in the separating gel...why? (reply: 8)
how to interpret native gel result - (reply: 1)
question on complexes - protein-ligand complex detection in native gels (reply: 1)
gel purification after restriction digestion - (reply: 3)
Wrong band size using precast gel - (reply: 4)
No bands at all in gel for PCR products - (reply: 5)
Broken gel apparatus--easy fix? - (reply: 9)
AFLP gel staining trouble - (reply: 1)
tris-tricine gel transfer - (reply: 3)
RNA extraction with smear after electrophoresis - (reply: 1)
ssDNA smear during gel shift - (reply: 3)
pcDNA3.1 & pBIG2i vectors appear "bigger" on the gel! :( - (reply: 1)
ChIP sonication Input Agarose gel - (reply: 1)
Gel retardation - (reply: 1)
Two bands on a denaturing gel - always two bands on a denaturing gel with double stranded substrate? (reply: 1)
Checking integrity of total RNA using gel? - (reply: 6)
Introduced to Gel Retardation Assay - Today in practical class, our lecturer instructed us in GRA (reply: 2)
separation of pcr products of similar sizes in agarose gel - (reply: 1)
RNA Gel Problems - RNA not showing up as bands in gel (reply: 6)
resolution of similar size products using agarose gel - heeeeeelp !!! =P (reply: 10)
Agarose gel concentration for genomic DNA - (reply: 5)
SDS-PAGE gel drying - (reply: 8)
purification by gel? - after a first round of PCR (reply: 2)
human RNA contaminated with bacterial RNA - give your opinion about a RNA gel (reply: 8)
how to melt agarose, and keep it melted? - (reply: 3)
Help Needed! DNA sample dispersed b4 electrophoresis - (reply: 2)
gel and gelatin - (reply: 1)
Gel shift assay follow by Western blot analysis - Has anyone try this before? (reply: 8)
Gel Electrophoresis DNA base pairs size - (reply: 1)
U-shaped smear on EMSA gel - (reply: 1)
How to stain /destain quickly SDS-PAGE gel? - your patents? (reply: 13)
SOS: Gel purification of small size DNA - (reply: 6)
agarose gel storage - (reply: 6)
Gel Shift assays - (reply: 3)
RNA gel problems - (reply: 2)
protein did not enter the gel on EMSA - (reply: 2)
Question about gel purification of PCR product - (reply: 1)
IP and 2D gel analysis - buffer composition (reply: 1)
2D electrophoresis - (reply: 1)
gel extraction problem - (reply: 6)
RNA Formaldehyde Gel problem - (reply: 5)
DNA Gel Extraction Problem - multiple bandings in gel extraction product (reply: 5)
Best Brand of Agarose? - (reply: 4)
Help with electrophoresis - Phosphate ester electrophoresis (reply: 1)
Smears on agarose gel, contamination? - (reply: 4)
Glycine in gel; fiber typing - (reply: 1)
Obtain larger dna fragments after digestion and gel cleaning - (reply: 2)
Genomic DNA on agarose gel - two bands of genomic DNA on agarose gel (reply: 2)
QIAquick Gel Extration Kit - (reply: 8)
Agarose / salmon sperm slurry - from upstate (reply: 5)
gel extraction of pcr products - (reply: 3)
do agarose gels expire? - (reply: 4)
Image processing of gel - (reply: 3)
non-specific bands in agarose gel - too many bands visible! (reply: 3)
Salmon sperm / agarose slurry - (reply: 3)
which agarose to use for pre-clearing - IP (reply: 1)
DNA concentration calculation from agarose gel - DNA concentration calculation (reply: 4)
shift of protein bands in SDS gel electrophoresis - where does it come from, can it be improved (reply: 2)
staining gels and western blot - (reply: 1)
agarose gel electrophoresis - (reply: 4)
best gel concentration and voltage - (reply: 3)
Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
how to apply molecular weight marker on 2D gels? - application marker for 2D (reply: 1)
Odd curve of Gel purified PCR product - (reply: 5)
gel purification - (reply: 1)
Polyacrilamide Gel - (reply: 2)
agrose Vs. PAGE - (reply: 3)
RNA electrophoresis problem - RNA not denatured (reply: 9)
gDNA on a gel? - (reply: 7)
Gel Extraction of DNA - (reply: 5)
EMSA/Gel Shift wells problem - Protein stuck in the wells of EMSA gel (reply: 10)
Problem when making PAGE gel - (reply: 7)
can i autoclave agarose gel with TAE buffer - (reply: 4)
Dry SDS-PAGE gels - methods, tips to safely dry protein gels (reply: 2)
problem with agarose gel? - (reply: 11)
Polyacrylamide gel not polymerize - (reply: 5)
Protein not penetrating the resolving part of the gel - (reply: 2)
RNA isolation - i want to show a gel (reply: 4)
Rhinohide polyacrylamide gel - (reply: 6)
Identification of the cut and uncut plasmid on gel - (reply: 4)
Gel extraxction question - (reply: 6)
Power setting for electrophoresis - (reply: 5)
2D electrophoresis of Plasma - (reply: 3)
Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
How to determine appropriate agarose % - (reply: 8)
Gel extraction and elution buffer - (reply: 4)
SDS-PAGE gel not polymerize - (reply: 11)
How to get sharp band on agarose gel? - (reply: 7)
Gel extraction problem - (reply: 15)
Sepharose or agarose in ChIP assay - (reply: 2)
Single cell gel electrophoresis (comet assay) - (reply: 1)
SDS-PAGE gel sticking - Gel sticking to the membrane (reply: 3)
problem with casting PAG - large number of persistent air bubbles in a gel (reply: 7)
DNA not entering the gel - (reply: 1)
can i store gel cut for dialysis - (reply: 3)
PCR product on gel as smear - DNA isolated from paraffin blocks why smear? (reply: 1)
Trouble with IEF gels - (reply: 2)
gel running problems - lanes are smeared lines on the gel (reply: 3)
DNA not entering the gel - (reply: 3)
southern blot - gel loading - (reply: 2)
DNA not entering the gel - (reply: 3)
Is my gel not polymerising fully and why......? - (reply: 1)
gel staining - (reply: 1)
Unspecific bands after gel purification, why? - (reply: 7)
in- gel trypsin digestion - (reply: 1)
DNA binding by RE causes bigger band than substrate in gel? - Restriction digest of 500bp frag. with BsaXI (reply: 4)
Ethidium Bromide POLL - Does anyone nuke their agarose w/ EtBr (reply: 7)
SDS gel not polymerize - (reply: 6)
SDS-PAGE gel problem - problems with the bottom zone of the gel (reply: 3)
streaks on 2D gels - (reply: 4)
1% formaldehyde gel bands - (reply: 1)
smear of loading buffer observed - SDS-PAGE gel (reply: 5)
Gel Supershift - to DTT, or not to DTT? (reply: 1)
can I freeze the gel slice for protein sequencing - (reply: 1)
Quick way of purifying DNA? - Preparative Gel is time consuming (reply: 3)
Agarose or acrylamide gel for DNA? - (reply: 6)
gels used for RNA detection - (reply: 3)
Ni-NTA problem - His-tag protein that never bound with Ni-NTA agarose (reply: 10)
No peak for gel filtration for soluble protein - Need help (reply: 2)
Agarose Gel Electrophoresis of plasmid DNA - Agarose Gel Electrophortesis of plasmid DNA (reply: 1)
gel migration - DNA gel migration problem (reply: 1)
Extrremely bright bands in the WELLs of PCR agarose gel - Bright and sharp bands in the PCR gel well (reply: 9)
In Gel Ligation - Anyone done it? (reply: 4)
Protein purification - Problem with his-tag protein gel filtration (reply: 5)
Agrose gel electrophoresis of bacterial RNA - 23srRNA and 16srRNA were not separated clearly (reply: 1)
Gel run of pGEX-4T - (reply: 4)
SDS lysate impurities - streaming on gel with SDS lysates (reply: 1)
Non toxic gel staining for highschool student work - Agarose gel stains (reply: 3)
Promega Gel Purification Kit - Cant get rid of ethanol (reply: 3)
Reuse TAE buffer and agarose gel? - yes or no... (reply: 22)
Protein isolation from acrylamide gels - (reply: 2)
Polymerization SDS Gel - (reply: 7)
Collagen gels not setting!! - (reply: 2)
DGGE and Smiling bands! - Side bands on my DGGE gel keep smiling! (reply: 2)
gel stain and gel extraction - crystal violet (reply: 1)
How imidazole can be washed off the Ni-NTA agarose beads - reuse of Ni-NTA agarose beads for His protein (reply: 2)
Electrophoresis - negative bands - (reply: 1)
gel analysis of large RNA products - (reply: 3)
Phenol red in agarose gel - (reply: 1)
affinity chromatography - sepharose and agarose (reply: 1)
Storage of 30% bis/acrylamide and SDS-PAGE gels - (reply: 8)
Erroneous agarose gel bands - (reply: 2)
Isolation restr. fragments from agarose gel by Qiagen kit - cloning fungal genomic DNA (reply: 1)
use of direct current for electrophoresis - (reply: 1)
EtBr in gel when run mRNA? - (reply: 2)
Agarose gel issue - (reply: 3)
Cells in collagen gel - Growing mammalian cells in collagen gel (reply: 1)
Why is my Agarose gel glowing? - (reply: 12)
Strange mobility behavior of PCR products on agarose gel - EB stained vs Gelstar stained (reply: 2)
clonning gel purification - (reply: 1)
Help: try ligation but see nothing from the gels - (reply: 9)
ISSR - electrophoresis - (reply: 2)
my bands spread as a line trough the gel - (reply: 3)
SDS gel troubleshoot - My proteins do not show up in the gel. (reply: 1)
Well adding Sybr green in a PCR mix - to detect quicker than by electrophoresis PCR prod (reply: 4)
DNA recovery from agarose gel---help!!! - (reply: 2)
Invitrogen platinum pfx enzyme not so fantastic - problems with pcr product gel migration (reply: 1)
Banding in agarose gel wells. - amplified fragment will not migrate! (reply: 7)
IEF gel problem - (reply: 1)
protein aggegation or? - protein in the wells of native gel (reply: 3)
Collagen Gel Contraction - Contracting gels using vitrogen collagen (reply: 1)
Immunoprecipitation and 2D gels - (reply: 1)
gel drying problem - asking for help (reply: 5)
Western blot for 2D gel - (reply: 1)
Large Fragment Gel Separation - (reply: 2)
about semi-quantity of PCR products on agarose gel - (reply: 2)
RNA electrophoresis - RNA electrophoresis (reply: 2)
In reference to low tm - I have smearing as well on a dna gel (reply: 2)
Formaldehyde Gel - Question about RNA gel (reply: 1)
how to do the antibody supershift through PAGE gel - (reply: 2)
Collagen gels - Problems with growth of endothelial cells on collagen gels (reply: 1)
2D gel streaking - 2Dgel streaking (reply: 3)
How to elute proteins from a SDS-PAGE gel? - (reply: 1)
Ethidium bromide DNA gel staining - rapidly fading bands (reply: 1)
qiaxII gel extraction kit.... - (reply: 1)
Probe preparation for gel shift - (reply: 1)
Plasmid DNA extraction - Extracted plasmid cannot be seen in agarose gel (reply: 9)
electrophoresis resolution - separate DNA of similar size - (reply: 10)
No 28s rRNA band in agarose gel? - (reply: 1)
polyacrylamide-agarose gel - (reply: 2)
Three bands after Ni agarosecolumn - (reply: 1)
Total RNA agaroseelectrophoresis - (reply: 1)
Zymmography gels - (reply: 1)
Electrophoresis - (reply: 3)
PAGE electrophoresis - (reply: 4)
electrophoretc mobility shiftassay - (reply: 1)
Low pH Native gel - (reply: 1)
a childish qustion about agrose gel electrophoresis - (reply: 2)
RNA electrophoresis - (reply: 1)
QIAquick gel extraction kit - high salt problem - QIAquick gel extraction kit - high salt problem (reply: 1)
Gel separation of two fragments of similar size - (reply: 1)
Fixing a gel - meaning? - (reply: 1)
electrophoresis buffer - when I should replace running buffer (reply: 1)
Protein extraction form gels - (reply: 2)
2 D electrophoresis - (reply: 2)
How to calculate the migration distance? - Gel Electrophoresis (reply: 2)
SDS-PAGE gel cracks - PAGE gel cracks after framing in cellophane (reply: 1)
Why is formaldehyde added to RNA gels? - (reply: 1)
Lyophilized or Silica gel dried tissue protocols - DNA protocols (reply: 1)
gel shift/supershift conditions - (reply: 2)
proteins electrophoresis - (reply: 2)
RNA on formaldehyde gel - (reply: 1)
QIAquick Gel Extraction Kit - (reply: 1)
phantom band in DNA purification - A new band appears after purifying DNA from agarose gels (reply: 1)
Ethidium bromide in formaldehyde gel? - (reply: 1)
How to make PCR using the gel solution - (reply: 1)