Protein A agarose for CHIP--pre-treatment is needed? - (Jan/08/2007 )
Hi, everybody,
I have been using protein A agarose from Roche for CHIP without any pre-treatment, and get aceptable result heretofore. But recently I found many CHIP protocols described the use of the pre-treated ones, such as the Protein A agarose/salmon sperm DNA from Upstate, to suppress the background signal. Does the use of pre-absorbed agarose beads can greatly improve the specifity?
Thank you very much.
-chycheng-
QUOTE (chycheng @ Jan 8 2007, 08:15 PM)
Hi, everybody,
I have been using protein A agarose from Roche for CHIP without any pre-treatment, and get aceptable result heretofore. But recently I found many CHIP protocols described the use of the pre-treated ones, such as the Protein A agarose/salmon sperm DNA from Upstate, to suppress the background signal. Does the use of pre-absorbed agarose beads can greatly improve the specifity?
Thank you very much.
I have been using protein A agarose from Roche for CHIP without any pre-treatment, and get aceptable result heretofore. But recently I found many CHIP protocols described the use of the pre-treated ones, such as the Protein A agarose/salmon sperm DNA from Upstate, to suppress the background signal. Does the use of pre-absorbed agarose beads can greatly improve the specifity?
Thank you very much.
The thing that treatment with carrier DNA or BSA can do is reduce background. If you're not having problems with high background then it's not an issue.
-KPDE-
QUOTE (chycheng @ Jan 9 2007, 04:15 AM)
Hi, everybody,
I have been using protein A agarose from Roche for CHIP without any pre-treatment, and get aceptable result heretofore. But recently I found many CHIP protocols described the use of the pre-treated ones, such as the Protein A agarose/salmon sperm DNA from Upstate, to suppress the background signal. Does the use of pre-absorbed agarose beads can greatly improve the specifity?
Thank you very much.
I have been using protein A agarose from Roche for CHIP without any pre-treatment, and get aceptable result heretofore. But recently I found many CHIP protocols described the use of the pre-treated ones, such as the Protein A agarose/salmon sperm DNA from Upstate, to suppress the background signal. Does the use of pre-absorbed agarose beads can greatly improve the specifity?
Thank you very much.
Dear chycheng,
Since I have trouble with SC protein A/G plus agarose (i.e. high background) please send me the catalog number of protein A agarose from Roche that you use.
Is it immobilized recombinant protein A gel suspension
11719408001 (1719408) 2 ml
11134515001(1134515) 5 ml
Thanks in advance
Grooya
-grooya-