dna ladder seperation in gel - (Apr/13/2008 )

hi all,
i use a 1kb plus dna ladder and when i run it on a 1% gel in TBE buffer, i get double bands or broad bands for the ladder.can someone suggest what i should do to get thinner bands. thanx.

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A 1.5% gel will separate the bands a little better and tighten them up more. If you need to run 1%, try running it a little slower for a longer time. Hope this helps

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thanx for the suggestion.i run the gel at 90v for 1 hour.the product description for the ladder gives a good seperation at 0.9%.does the thickness of the gel anything to do with this problem.

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Try adding less marker and running slower.

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what is the optimum concentration of ladder to get clear bands. when i run the gel at 70v the bands above 3kb in the ladder seem to be clear and thin,whereas below that it looks broad and dull.

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