sticky and blunt DNA on gel migration - (Aug/29/2007 )
Hi everyone,
I would like to ask whether there's any difference in agarose gel migration for DNAs with sticky ends and blunt-end (resulted by restriction cleavage). If yes, how is the form of DNA affecting the migration? Thank you very much in advance.
Regards,
haohao
I think the differences is not significant.
I would like to ask whether there's any difference in agarose gel migration for DNAs with sticky ends and blunt-end (resulted by restriction cleavage). If yes, how is the form of DNA affecting the migration? Thank you very much in advance.
Regards,
haohao
you will get same band size for both DNA with sticky ends and blunt end
I dont think there should be any difference in DNA migration in the gel.
Unless you run very very small DNA fragments in an acrilamide gel, you will never see a difference.
Back in the dim, dark past, when lambda phage was still commonly used for markers, you were recommended to occasionally re-heat the sample before use. The two terminal repeat sequences showed the propensity to re-anneal, so you'd lose two fragments, and make one larger one. But if you're only talking about 4-base overhangs, I think you'd be hard pressed to see any difference in agarose, although CE might, maybe show a slight difference in migration time.