Help! low molecule weight peptides electrophoresis! - (Apr/08/2008 )

Hello，everybody！
I need to detect a small peptides with MW:4900~ by electrophoresis, I have surveyed some literatures, Ticine-SDS-PAGE with gradient gel does not woek, maybe urea can improve resolution, but I don't know about the principle clearly, I hope anybody who knows to give me a explanation in detail, thanks!! that's very nice if you can tell me the role of Tricine in the Ticine-SDS-PAGE. thank you very much!!!

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Have you read this article about tricine vs glycine? It might help clarify things for you. Also if you're not using PVDF membranes (assuming you are doing westerns), then your small peptides will blow through.

http://www.nature.com/nprot/journal/v1/n1/...rot.2006.4.html

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nice reference, smu2.

we use small pore nitrocellulose for westerns of small proteins and peptides. works fine.

stevensi, what are you seeing (or not seeing) in your gel? we run gradient tris-tricine gels and get excellent resolution.

is your protein a glycoprotein? glycoproteins give fuzzy bands.

the addition of urea (4-8M) helps sharpen bands (the reference smu2 provided uses 6M).

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