Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

I need your help regarding HAEMOGLOBIN ELECTROPHORESIS - (Feb/02/2007 )

HIIIIII.
I have been assigned the job of Haemoglobin Electrophoresis which I have no idea about mellow.gif .I know that it could be done by modifying your original SDS-PAGE protocol but still don't know what these modifications are and how to go about the whole process.Can you people help me carrying out this? smile.gif

-raaj-

exactly how do you want to analyze the hemoglobin?

you can run sds-page and denature the hemoglobin.

you can run on native page, at high neutral or low pH or cellulose acetate strips to see subtypes of hemoglobin without denaturing the hemoglobin.

-mdfenko-