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How can I dissolve 6%polyacrylamide gel? - (Nov/22/2006 )

I'm trying to obtain a 260bp DNA band from 6% polyacrylamide gel without using Gel extraction Kit. It's hard to dissolve gel slice (i tried to boil it in water for 1 hour and in QG buffer of QIAgen but I wasn't successful). I found some advices on Internet but I don't know which I can apply, can someone help me to choose or give me another advices:
1.Incubate the slice in 50ul TE overnighr at 37 deg
2.Incubate it in elution buffer at 37 deg with saking for several hours
3.Boil it in phenol/chloroform/isoamyl alcohol (25:54:1) for 10 min
I just need DNA to reamplify, so can I reamplify immediately after I dissolve the slice?
I'm looking foward some answers, thank you very much!!!!

-bio_VN-

If you need a PCR template, you can simply put a pipet tip into the band, swish it around in the PCR tube, and go. Vanishingly small amounts of template are required.

-phage434-

some simple electroelution procedure should be done using becher falcon 50ml tube small agarose and 2cables... and is safer for DNA than boilong in PCI i think

-fred_33-