Lyophilized or Silica gel dried tissue protocols - DNA protocols (Jul/13/2002 )
I am looking for good protocols to extract DNA from lyophilized or silica gel dried plant tissue. I will be doing AFLPs on these samples for a mapping project and so the quality needs to be good. Any suggestions?
-bgardunia-
bgardunia,
There is a paper that just came out in the latest issue of BioTechniques (vol 31(1) pgs 132-140) that used dried plant tissue in real-time PCR. They used Puregene for this.
AronD
-AronD-