bands/dyes don't move on gel - (Oct/16/2007 )
I'm very surprised, i'm running dna on agarose gel but the bands don't move as evident from the dyes. Even in UV bands stay on top in wells. The power is on. The gel is made according to protocol so what could be happeneing here?
It sounds as if no current is flowing. Is there buffer in the electrophoresis chamber? Are the leads connected? Is the power supply plugged in?
The power is on, the chamber has buffer and the leads are connected. Could it be because i left for long time to soldify as in being over soldified? Now i'm making a new gel but i don't know what to change because its the way i make and run every time. It could be because i heated the agar+tae too much to dissolve it
If you can't see any bubble showing up on one of the electrodes, then the connection has problem.
nah, you have to boil the agar for it to dissolve. heating won't be the problem.
how do you make the gel?
are you using 1X tae or 10X tae?
what percentage agarose do you use?
how much DNA are you putting in each well?
have you cleaned the combs before using them?
Vetticus
nah, you have to boil the agar for it to dissolve. heating won't be the problem.
how do you make the gel?
are you using 1X tae or 10X tae?
what percentage agarose do you use?
how much DNA are you putting in each well?
have you cleaned the combs before using them?
Vetticus
OR is the DNA tooo heavy?! what's the size..? if its too heavy and yr gel % high.. the DNA won't move. I'm assuming.. u do see a current and voltage change through yr run.. and everything else is fine too.. the buffer, the unit?
so tell us the size and gel%!!
G'luckk!
Dye is also not moving.
Will the movement of dye also be influenced by size of DNA and the % of Agarose? 
Like genehunter asked, do you see bubbles or not?
I was thinking of two things. The first (already said a lot) that there is no current (indeed check for bubbels), or your TAE/TBE concentration is too high (maybe forget to dilute?) That also happended to me once and I got this problem 
ohh.. yeah.. if the dye isn't moving too.. check the current.. voltage.. etc.. bubbles too!!
Check bubbles, certainly! Also check if buffer is correctly diluted or better, do the dilution yourself. Don't forget the buffer in the gel and in the chamber must be at the same concentration, otherwise it won't run!