Smeary DNA band on agarose gel - (Oct/17/2006 )

What causes of band smear formation?

Thx !

-hsbc-

could you precise your question?
what kind of gel, what kind of sample, running conditions, optionnaly you could also send a picture of your smeared bands.

-Missele-

more details please.

-scolix-

QUOTE (Missele @ Oct 17 2006, 04:05 PM)

could you precise your question?
what kind of gel, what kind of sample, running conditions, optionnaly you could also send a picture of your smeared bands.

Thanks for reminding.
It is 2% agarose gel, runs DNA (GM Food sample) at 110V for about 15 mins.
Sorry i can't show you a picture coz i don't have any in my computer.

Any more information provided is welcome.

-hsbc-

sample degradation by DNase could result in a smear, or the presence of RNA also, if you forgot to treat with RNase.
If it's a degradation, you see a smear starting at your band of interest, and going down, but not on the top side of your band.

-Missele-

if its RNA, it would run at much lower range.

and for DNA, as missele suggested.

-scolix-