How to elute protein from SDS-PAGE gels - (Apr/09/2007 )
How to elute proteins from SDS gels, any protocol
-coffee-
you can electroelute.
or
the easiest way is to homogenize the excised band in a little buffer and centrifuge to pellet the gel and collect the supernate.
-mdfenko-
QUOTE (coffee @ Apr 9 2007, 02:22 PM)
How to elute proteins from SDS gels, any protocol
is it fixed? so electroelution should be successful optionally w/o mincing the gel;
-The Bearer-
QUOTE (coffee @ Apr 9 2007, 05:22 AM)
How to elute proteins from SDS gels, any protocol
Take a look here
manual Gebaflex
I hope it helps
-circlepoint-