Normal agarose gel vs formaldehyde agarose gel - (May/23/2008 )

Hi everybody,


I would to ask few questions regarding to those figures. Figure 1 and 2, I runned 30 mers oligonucleotide in 1.5% agarose gel (figure 1) and Formaldehyde gel (figure 2) in different concentration. I would like to know why there are double bands in figure 2. In contrast they appear single band in figure1. Is it because of Formaldehyde gel would give double band to oligonucleotide? May i know, is it normal to get double band if we use FA gel for DNA sample? Please comment about this double band in figure 2..... please.....

And secondly, the question is based on figure 3, I heard that 28s (first band) should be brighter than 18s (second band) in 2:1 ratio. Is it right? because in my gel is different (figure3) 18s (second band) is brighter than 28s (first band).

Please someone guide me?

Shilina

Hi everybody,


I would to ask few questions regarding to those figures. Figure 1 and 2, I runned 30 mers oligonucleotide in 1.5% agarose gel (figure 1) and Formaldehyde gel (figure 2) in different concentration. I would like to know why there are double bands in figure 2. In contrast they appear single band in figure1. Is it because of Formaldehyde gel would give double band to oligonucleotide? May i know, is it normal to get double band if we use FA gel for DNA sample? Please comment about this double band in figure 2..... please.....

And secondly, the question is based on figure 3, I heard that 28s (first band) should be brighter than 18s (second band) in 2:1 ratio. Is it right? because in my gel is different (figure3) 18s (second band) is brighter than 28s (first band).

Please someone guide me?

Shilina