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SDS in gel or not? - (Sep/24/2008 )

SDS in gel+running buffer vs SDS in running buffer only (Anderson gel???)
Does it worth to try both?

-kingswill-

QUOTE (kingswill @ Sep 24 2008, 08:26 AM)
SDS in gel+running buffer vs SDS in running buffer only (Anderson gel???)
Does it worth to try both?


if you're doing a regular western, you put SDS in both.

-smu2-

SDS only in running buffer is also working, but I agree with smu2, for western blotting I would also do both. It is no big deal. No big time saving. Or money.

-biomaus-