purification by gel? - after a first round of PCR (Oct/13/2005 )
I'm trying a mutagenesis by PCR with 4 oligos ... after the first round they say "gel purify fragment" what am i supposed to do? put all my reaction in an agarose gel then cutting the gel to retrieve my DNA?
thank you very much!
-SMH-
QUOTE (SMH @ Oct 13 2005, 09:58 AM)
I'm trying a mutagenesis by PCR with 4 oligos ... after the first round they say "gel purify fragment" what am i supposed to do? put all my reaction in an agarose gel then cutting the gel to retrieve my DNA?
Yes. -HomeBrew-
ok thanx!
-SMH-